Method for detecting influence of heated incombustible cigarette total particle matters on cellular reactive oxygen secretory volume
A heat-not-burn, cell-active technology, applied in biochemical equipment and methods, measurement devices, microorganisms, etc., can solve the problems of no unified standard method, time-consuming and labor-intensive
Inactive Publication Date: 2019-04-09
CHINA TOBACCO YUNNAN IND
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Problems solved by technology
However, there are a large number of primary formulations, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use biological testing technology to screen the primary formulations of the product to obtain the results of reducing the adverse feelings of the human body? Product formula is still in the exploratory stage, and there is no unified standard method
Method used
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Embodiment 1
[0030] Prepare 3 kinds (1#, 2#, 3#) of heat-not-burn cigarettes with total particulate matter captured by Cambridge filter as samples, and test its effect on the secretion of reactive oxygen species in cells.
[0031] The specific detection process includes the following steps:
[0032] (1) Sample pretreatment: using a linear smoking machine, under the suction conditions of a suction volume of 55.0mL, a duration of 3s, and a suction frequency of 30s, continuously suck 10 mouthfuls, and use a Cambridge filter with a diameter of 44mm Capture, weigh the mass of the smoke trap before and after smoking, and calculate the mass mTPM of the total particulate matter in the heat-not-burn cigarette according to formula (1);
[0033] mTPM=m 1 -m 0 (1)
[0034] In the formula:
[0035] m 0 - the mass of the smoke trap before suction, in milligrams (mg);
[0036] m 1 - the mass of the smoke trap after suction, in milligrams (mg);
[0037] After the capture is completed, put the cap...
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Abstract
The invention discloses a method for detecting the influence of heated incombustible cigarette total particle matters on cellular reactive oxygen secretory volume. The method comprises the following steps: firstly, pre-treating a sample; secondly, preparing human nasal epithelial cell single cell suspension; thirdly, calculating the cell concentration of the human nasal epithelial cell single cellsuspension; fourthly, inoculating the human nasal epithelial cell single cell suspension; fifthly, exposing a test material; sixthly, loading a probe; seventhly, determining the sample; eighthly, calculating the reactive oxygen level. By effectively treating the sample, optimally setting the detection dose and correctly selecting target cells, the method can accurately detect the influence of theheated incombustible cigarette total particle matters on the cell reactive oxygen secretory volume and further can effectively evaluate oxidative damage of the heated incombustible cigarette total particle matters on cells.
Description
technical field [0001] The invention belongs to the technical field of safety biological effect evaluation of tobacco products, and in particular relates to a method for detecting the influence of total particulate matter in heat-not-burn cigarettes on the secretion of active oxygen in cells. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. Heat-not-burn cigarette products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to shift from traditional cigarette products. [0003] Heat-not-burn cigarettes are characterized by heating tobacco rather than burning it, providing consumers with a certain tobacco characteristic experience. In the in...
Claims
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IPC IPC(8): C12Q1/02
CPCC12N2503/00G01N33/5014G01N33/5044
Inventor 管莹李雪梅朱东来张霞韩敬美何东沅张伟高茜徐玉琼汤建国
Owner CHINA TOBACCO YUNNAN IND