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SNP molecular markers related to sheep tail types and applications thereof

A technology of molecular markers and sheep, applied in the field of genetic biology, can solve the problems of lack of SNP molecular markers and achieve the effect of maintaining stability

Active Publication Date: 2018-03-23
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the lack of SNP molecular markers directly related to fat tail / thin tail, the present invention provides a SNP molecular marker for detecting sheep tail fat

Method used

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  • SNP molecular markers related to sheep tail types and applications thereof
  • SNP molecular markers related to sheep tail types and applications thereof
  • SNP molecular markers related to sheep tail types and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Using the SNP molecular marker Chr13-49051930 to identify sheep tail type.

[0036] The test subjects were small-tailed Han sheep and Dorper sheep hybrid sheep (100), Ujumqin and Australian Merino hybrid sheep (100), Luxi black-headed sheep (100), small-tailed Han sheep (100), Tan sheep (50), Ujimqin sheep (50), Hu sheep (50), Sunit sheep (50), Cele black sheep (50), Bayinbulak sheep (50 ), one month old.

[0037] The specific primers for SNP molecular markers are:

[0038] Forward primer: 5'-TACGACCCTTTCATTGGGGG-3'

[0039] Reverse primer: 5'-ACAGTCACAGGTGGTGCAAT-3'

[0040] (1) Collect 5 mL of anterior vena cava blood from sheep, and extract DNA by phenol-chloroform method;

[0041] (2) Using the genomic DNA of the sheep to be tested as a template, PCR amplification was carried out using specific primers for SNP molecular markers. The amplification system was shown in Table 1, and the amplification procedure was shown in Table 2 to obtain PCR products; ...

Embodiment 2

[0049] Example 2 Using the SNP molecular marker Chr13-49132297 to identify sheep tail type.

[0050] The test subjects were small-tailed Han sheep and Dorper sheep hybrid sheep (100), Ujumqin and Australian Merino hybrid sheep (100), Luxi black-headed sheep (100), small-tailed Han sheep (100), Tan sheep (50), Ujimqin sheep (50), Hu sheep (50), Sunit sheep (50), Cele black sheep (50), Bayinbulak sheep (50 ), one month old.

[0051] The specific primers for SNP molecular markers are:

[0052] Forward primer: 5'-aatggggacacattttccaa-3'

[0053] Reverse primer: 5'-tggctcttggtgagtggtta-3'

[0054] (1) Collect 5 mL of anterior vena cava blood from sheep, and extract DNA by phenol-chloroform method;

[0055] (2) Using the genomic DNA of the sheep to be tested as a template, PCR amplification was carried out using specific primers marked by SNP molecules. The amplification system was shown in Table 3, and the amplification procedure was shown in Table 4 to obtain PCR products; El...

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Abstract

SNP molecular markers for detecting sheep tail types are provided. The polymorphism of one of the SNP molecular marker at the 49051930 site of the number 13 chromosome is C / G, and the polymorphism ofthe other SNP molecular marker at the 49132297 site of the number 13 chromosome is A / G. The SNP molecular markers can be used for identifying the sheep tail types and breeding thin-tail type sheep.

Description

technical field [0001] The invention belongs to the field of genetic biology, and in particular relates to a SNP molecular marker related to sheep tail type and its application. Background technique [0002] In sheep, the morphology of the tail is divided into fat tail and thin tail. The fatty tail is large and contains a lot of fat inside, while the thin tail is small and contains little fat. [0003] Tail fat is a unique way for sheep to store energy. In spring and autumn, grasslands are rich in vegetation. Sheep eat a lot of food, convert energy into fat and store it on their tails, and form tail fat. The stored fat provides energy to survive the cold winter. However, as people's demand for mutton is increasing, the demand for tail fat is less and less, and tail fat occupies a large number of functions, resulting in a large amount of energy waste, which limits intensive breeding and is not conducive to the development of the mutton sheep industry. develop. However, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 潘章源吕慎金李富宽储明星王慧
Owner LINYI UNIVERSITY