Quorum sensing inhibitors for biofilm inhibition

A biofilm and hull technology, applied in biocides, animal repellents, plant growth regulators, etc., can solve problems such as unsatisfactory

Inactive Publication Date: 2018-03-23
LIFE MATTERS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing methods rely primarily on coating devices and immersing surfaces with protective coatings, but there are no satisfactory methods available for treating medically important biofilms

Method used

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  • Quorum sensing inhibitors for biofilm inhibition
  • Quorum sensing inhibitors for biofilm inhibition
  • Quorum sensing inhibitors for biofilm inhibition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 Biofilm inhibition properties of NN and DIV.

[0087] Biofilms of P. aeruginosa and A. baumannii grown on glass slides were determined using confocal scanning laser microscopy (CSLM) (Figure 1). Correspondingly, the densities of biofilms before and after NN and DIV treatments were measured. Both treatments caused a decrease in the density of attached cells, although NN was most effective for Pseudomonas aeruginosa. DIV and NN treatments had little effect on the density of Acinetobacter baumannii. Both treatments caused negligible bacterial mortality as they were not different from the controls.

[0088] The dynamic growth conditions in the flow cell system are considered to be representative of the real conditions in humeral bone tissue, where pathogens thrive in an enriched environment. Studies of the effect of inhibitor compounds on bacteria in terms of structured biofilm disruption showed that both compounds had similar broad anti-biofilm effects on P. a...

Embodiment 2

[0091] Example 2 Biofilm inhibitory properties of 3,3'-diindolylmethane (DIM)

[0092] In addition, we tested the antibiofilm properties of the well-studied anticancer compound 3,3'-diindolylmethane (DIM), a metabolite found in cruciferous vegetables. The inhibitory effect of DIM on biofilm formation was studied with different clinical pathogens under static conditions. Biofilms formed by Acinetobacter baumannii and Pseudomonas aeruginosa were further tested in a dynamic flow cytometry system, where biofilm inhibition levels of 86% and 76%, respectively, were obtained. The combined treatment with tobramycin and DIM showed a significant percent inhibition of biofilm formation of 94%, indicating almost complete eradication of bacteria. In addition, the results also indicated that DIM could potentially inhibit the secretion of unique virulence factors of Pseudomonas aeruginosa. Further testing of hypothesized synergistic effects obtained by combining conventional antibiotics wi...

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Abstract

A compound selected from 2-(indolin-2-yl)-1H-indole, di(1H-indol-3-yl)methane and 1,1'- biindole, or a combination thereof, for use in inhibiting biofilm formation by bacteria on a surface or disrupting existing biofilm on a surface is provided.

Description

technical field [0001] The present invention generally relates to preventing biofilm formation and disrupting existing biofilms. Background technique [0002] In general, bacteria can exist as solitary, independent cells (planktonic), or can be organized into sessile aggregates. The latter form is often referred to as the biofilm growth phenotype. Acute infections often involve planktonic bacteria, which are often treatable with antibiotics; whereas infections involving biofilm-resident bacteria often turn out to be incurable and develop into a chronic state. It is estimated that most bacterial infections in humans are biofilm-associated and approximately 50% of nosocomial infections are indwelling device-associated. [0003] The ability of many bacteria to adhere to surfaces and form biofilms is critical to industries including transportation, energy, water, food (e.g. dairy, fish, poultry, meat and ready-to-eat food processing), oil drilling, paper manufacturing, maricul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61P31/04C07D209/04C07D209/10
CPCA61K45/06A61K31/7036A61K31/404A61P31/04A61L29/16A61L31/16A61L2300/204A61L2300/404A61K2300/00A01N43/38A61L29/08A61L31/08
Inventor 阿里尔·库斯马洛罗伯特·S·马克斯卡琳娜·高乐伯格
Owner LIFE MATTERS LTD
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