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A vaccine composition, its preparation method and application

A vaccine composition and culture technology, applied in the field of vaccine compositions, can solve the problems of lack of co-infection combined vaccine, and achieve the effects of good immune protection, good immunogenicity and resistance to infection

Active Publication Date: 2021-04-06
PULIKE BIOLOGICAL ENG INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Vaccine prevention is an effective way to prevent and control these two diseases, but there is a lack of combined vaccines against the co-infection of these two pathogens in the prior art, especially the lack of combined vaccines against the co-infection of Newcastle disease epidemic strains and Avian bacillus paragallinia pathogens

Method used

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  • A vaccine composition, its preparation method and application
  • A vaccine composition, its preparation method and application
  • A vaccine composition, its preparation method and application

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Experimental program
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Effect test

Embodiment approach

[0016] As an embodiment of the present invention, in the vaccine composition of the present invention, the antigen of the gene VII Newcastle disease virus strain or its culture is an inactivated whole virus antigen.

[0017] The NDV N7a strain is a gene type VII Newcastle disease virus strain, which is a Newcastle disease virus strain in which the F cleavage site 112R-R-Q-K-R-F117 of the gene type VII Newcastle disease epidemic wild strain is mutated into an attenuated cleavage site 112G-R-Q-G-R-L117.

[0018] "Vaccine composition" refers to a pharmaceutical composition containing the immunogenicity of Avibacterium paragallinarum and Newcastle disease virus type VII, which can induce, stimulate or enhance the immune response of chickens against Avibacterium paragallinarum and Newcastle disease virus . The vaccine composition can include the attenuated live vaccine, inactivated vaccine, subunit vaccine or synthetic peptide vaccine of immunizing amount of Avian bacillus paragall...

Embodiment 1

[0060] The preparation of embodiment 1 Avian bacillus paragallinarum antigen

[0061] 1. Primary seed propagation

[0062] Streak inoculated strains on chicken broth agar plate in 5%-10% CO 2 Cultivate at 37°C for 18-24 hours in the environment, select a suitable number of typical translucent dewdrop-like bacterial lawns (colonies) with strong fluorescence, inoculate the bacterial lawns into the yellow sac of chicken embryos (6-7 days old), and place them at 37 Continue to incubate at ℃, collect the yolk liquid of chicken embryos that died within 30 hours, and use them as first-class seeds after passing the pure inspection.

[0063] 2. Secondary seed propagation

[0064] Take the egg yolk fluid of infected chicken embryos, streak and inoculate the chicken broth agar plate, culture at 37°C for 18 to 24 hours in an environment containing 5% to 10% CO2, select typical colonies with strong fluorescence and inoculate them in the broth medium, place Cultivate at 37°C and 180rpm f...

Embodiment 2

[0069] The preparation of embodiment 2 chicken Newcastle disease antigen

[0070] Get chicken Newcastle disease virus N7a strain, do appropriate dilution with sterilized physiological saline (10 -4 or 10 -5 ) to inoculate 10-11-day-old susceptible chicken embryos, 0.1ml per embryo, and continue to incubate at 37°C after inoculation. Select dead and surviving chicken embryos 48 to 120 hours after inoculation, harvest the allantoic fluid, and measure the virus content, which is 10 8.0 EID 50 / 0.1ml. Add formaldehyde solution (v / v) with a final concentration of 0.1%, put it at 37°C for inactivation, stir once every 4-6 hours, inactivate for 16 hours, and set aside after complete inactivation.

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Abstract

The present invention provides a kind of vaccine composition, and this vaccine composition comprises A type A poultry bacteria antigen, C type A poultry bacteria antigen, Newcastle disease virus strain N7a strain or its culture antigen, and pharmaceutically acceptable The carrier, the vaccine composition of the present invention can provide comprehensive protection against genotype VII and classic Newcastle disease virus; and the antigens cooperate well, the immune effect is excellent, and even the two antigens can promote each other and act synergistically. The immunization effect of the double vaccine of the invention is better than that of the single vaccine, and the safety is better, avoiding the immune paralysis caused by multiple immunizations, and reducing the stress on the corresponding flocks.

Description

technical field [0001] The invention relates to a vaccine composition, a preparation method and application thereof, belonging to the field of veterinary biological products. Background technique [0002] Chicken infectious rhinitis (Infectious Coryza) is an acute upper respiratory infectious disease caused by Haemophilus paragallinarum. The main clinical features are facial edema and runny nose. And the increase of poor growth and elimination rate of broiler chickens has caused greater economic losses to the poultry industry. [0003] Chicken Newcastle disease (New Castle disease) is a kind of highly contagious infectious disease caused by paramyxovirus, acute septicemia infectious disease, is commonly called as " chicken plague ", namely so-called Asian chicken plague. The disease can occur throughout the year, especially in cold and climate-changing seasons. Chickens of various ages can be infected, among which 20 to 60 days are the most susceptible and have a higher mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/14
CPCA61K39/102A61K39/12A61K2039/552A61K2039/55505A61K2039/70C12N2760/18134A61K2300/00
Inventor 田克恭胡欢鑫孙进忠张许科
Owner PULIKE BIOLOGICAL ENG INC
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