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A method for simultaneously detecting multiple progestogens in environmental water

A technology for detecting environment and progesterone, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the recovery rate and repeatability cannot be guaranteed, and achieve the effects of wide application range, improved selectivity, and high effective combination

Active Publication Date: 2020-03-10
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a new type of sample pretreatment technology, SPME technology continues to expand with its application fields, but some limitations of its extraction fiber itself lead to new problems, and the recovery rate and repeatability cannot be guaranteed.

Method used

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  • A method for simultaneously detecting multiple progestogens in environmental water
  • A method for simultaneously detecting multiple progestogens in environmental water
  • A method for simultaneously detecting multiple progestogens in environmental water

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A method for simultaneously detecting multiple progestogens in environmental water bodies, wherein the progestogens are respectively mifepristone, chlormadinone, medroxyprogesterone, drospirenone, progesterone, norethindrone, norethisterone, and Hydroprogesterone, levonorgestrel, norethindrone, progesterone-d9, norethindrone-d6. After the sample to be tested is pretreated by the new solid-phase extraction adsorbent Oasis PRiME HLB, it is detected by UPLC-MS / MS, including the following steps:

[0038] (1) Water sample pretreatment

[0039]The influent water, effluent water and river water of the sewage treatment plant were selected as water samples. The two water sources have a high human contact rate and are more representative. The collected water samples are subjected to three-stage filtration with filter membranes with three different pore sizes of 10 μm, 1 μm and 0.45 μm, and the filtered water samples are accurately measured. Among them, the influent water sample ...

Embodiment 2

[0053] A method for simultaneously detecting multiple progestogens in environmental water bodies, wherein the progestogens are respectively mifepristone, chlormadinone, medroxyprogesterone, drospirenone, progesterone, norethindrone, norethisterone, and Hydroprogesterone, levonorgestrel, norethindrone, progesterone-d9, norethindrone-d6. After the sample to be tested is pretreated by the new solid-phase extraction adsorbent Oasis PRiME HLB, it is detected by UPLC-MS / MS, including the following steps:

[0054] (1) Water sample pretreatment

[0055] The influent water, effluent water and river water of the sewage treatment plant were selected as water samples. The two water sources have a high human contact rate and are more representative. The collected water samples are subjected to three-stage filtration with filter membranes with three different pore sizes of 10 μm, 1 μm and 0.45 μm, and the filtered water samples are accurately measured. Among them, the influent water sample...

Embodiment 3

[0069] A method for simultaneously detecting multiple progestogens in environmental water bodies, wherein the progestogens are respectively mifepristone, chlormadinone, medroxyprogesterone, drospirenone, progesterone, norethindrone, norethisterone, and Hydroprogesterone, levonorgestrel, norethindrone, progesterone-d9, norethindrone-d6. After the sample to be tested is pretreated by the new solid-phase extraction adsorbent Oasis PRiME HLB, it is detected by UPLC-MS / MS, including the following steps:

[0070] (1) Water sample pretreatment

[0071] The influent water, effluent water and river water of the sewage treatment plant were selected as water samples. The two water sources have a high human contact rate and are more representative. The collected water samples are subjected to three-stage filtration with filter membranes with three different pore sizes of 10 μm, 1 μm and 0.45 μm, and the filtered water samples are accurately measured. Among them, the influent water sample...

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Abstract

The invention discloses a method for simultaneously detecting a plurality of progestogen in an environment water body, which belongs to the field of analysis of an organic matter in environment. The method comprises the following five steps of sample pretreatment, enrichment condensation of a novel solid-phase extraction adsorbent Oasis PRiME HLB, determination of progestogen concentration throughUPLC-MS / MS, drafting of a standard curve, and computer determination. The novel solid-phase extraction adsorbent Oasis PRiME HLB is taken as a pretreatment technology of an environment water sample,selectivity of a to-be-detected object is increased, enrichment multiple is greatly increased, matrix effect is reduced, a superhigh liquid phase-mass spectrometer is used as a detection quantification tool, 11 types of progestogen in the water environment can be simultaneously determined, comparing with a conventional detection mode, effective combination of high selectivity and high sensitivityof progestogen can be realized, and the method has the advantages of fast analysis speed and wide usage scope.

Description

technical field [0001] The invention belongs to the technical field of organic matter analysis in environmental water bodies, and in particular relates to a method for simultaneously detecting multiple progestogens in environmental water bodies. Background technique [0002] In the past half century, progesterone has caused serious harm to the natural environment on which humans and wild animals depend, and has become an urgent problem of environmental pollution worldwide, which has aroused widespread concern. As a common endocrine disruptor, progesterone can accumulate in the environment and the human body for a long time, metabolizes slowly, and can be transferred to various environmental media such as the atmosphere, soil, and water sediments through diffusion and convection. Once it enters the body, it can not only disrupt the normal synthesis and metabolism of endocrine hormones, affect growth and sex differentiation, but also cause developmental abnormalities and repro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08
CPCG01N30/02G01N30/06G01N30/08G01N2030/045G01N2030/062
Inventor 任洪强于清淼耿金菊吴刚
Owner NANJING UNIV