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A method for evaluating the safety of recombinant coxsackievirus b3 injection

A coxsackie virus and evaluation method technology, applied in the field of safety evaluation of recombinant coxsackie virus B3 injection, can solve the error of the evaluation method of recombinant coxsackie virus B3 injection safety, recombinant coxsackie virus Problems such as B3 injection error evaluation, to achieve the effect of reducing waste and simple operation steps

Active Publication Date: 2020-06-12
WUHAN BOWEIDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Recombinant coxsackievirus B3 injection needs to be tested and evaluated for its safety before use. However, in the prior art, there are large errors in the evaluation method for the safety of recombinant coxsackievirus B3 injection, which is easy to cause Misassessment of Recombinant Coxsackievirus B3 Injection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for evaluating the safety of recombinant coxsackievirus B3 injection, comprising the following steps:

[0017] S1, culture medium preparation, prepare multiple petri dishes, and then carry out disinfection treatment on multiple petri dishes by disinfection reagent respectively, after disinfection is completed, put cells and nourishment for feeding cells respectively in multiple petri dishes, and each cell It does not contain viruses that may be infectious or pathogenic to humans, and the disinfectant is medical alcohol;

[0018] S2. Virus inoculation, inoculating the recombinant Coxsackievirus B3 injection into multiple culture dishes respectively, and determining whether there is virus in the cells in the multiple culture dishes after a certain period of time;

[0019] S3, prepare the initial preparation, put the cells of recombinant Coxsackievirus B3 in S2 into the aqueous buffer, the osmolality of the aqueous buffer is 100mOSM / L, and the aqueous buffer conta...

Embodiment 2

[0023] A method for evaluating the safety of recombinant coxsackievirus B3 injection, comprising the following steps:

[0024] S1, culture medium preparation, prepare multiple petri dishes, and then carry out disinfection treatment on multiple petri dishes by disinfection reagent respectively, after disinfection is completed, put cells and nourishment for feeding cells respectively in multiple petri dishes, and each cell It does not contain viruses that may be infectious or pathogenic to humans, and the disinfectant is medical alcohol;

[0025] S2. Virus inoculation, inoculating the recombinant Coxsackievirus B3 injection into multiple culture dishes respectively, and determining whether there is virus in the cells in the multiple culture dishes after a certain period of time;

[0026] S3, prepare the initial preparation, put the cells of recombinant Coxsackievirus B3 in S2 into the aqueous buffer, the osmolality of the aqueous buffer is 120mOSM / L, and the aqueous buffer conta...

Embodiment 3

[0030] A method for evaluating the safety of recombinant coxsackievirus B3 injection, comprising the following steps:

[0031] S1, culture medium preparation, prepare multiple petri dishes, and then carry out disinfection treatment on multiple petri dishes by disinfection reagent respectively, after disinfection is completed, put cells and nourishment for feeding cells respectively in multiple petri dishes, and each cell It does not contain viruses that may be infectious or pathogenic to humans, and the disinfectant is medical alcohol;

[0032] S2. Virus inoculation, inoculating the recombinant Coxsackievirus B3 injection into multiple culture dishes respectively, and determining whether there is virus in the cells in the multiple culture dishes after a certain period of time;

[0033] S3, prepare the initial preparation, put the cells of recombinant Coxsackievirus B3 in S2 into the aqueous buffer, the osmolality of the aqueous buffer is 135mOSM / L, and the aqueous buffer conta...

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PUM

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Abstract

The invention relates to the technical field of recombinant coxsackievirus B3, in particular to a method for evaluating safety of a recombinant coxsackievirus B3 injection. The method comprises the following steps: preparation of a culture medium, virus inoculation, preparation of a starting preparation, inactivation with the starting preparation as well as inactivation evaluation. The recombinantcoxsackievirus B3 is injected into a cell and whether an antibody can be generated in the cell is determined, so that the influence on the safety of the human body after the recombinant coxsackievirus B3 is injected into a human body is determined, a detection result is directly displayed in front of a worker, the method adopts simple operation steps, and time waste is reduced.

Description

technical field [0001] The invention relates to the technical field of recombinant coxsackievirus B3, in particular to a method for evaluating the safety of recombinant coxsackievirus B3 injection. Background technique [0002] Recombinant coxsackievirus B3 injection needs to be tested and evaluated for its safety before use. However, in the prior art, there are large errors in the evaluation method for the safety of recombinant coxsackievirus B3 injection, which is easy to cause Error assessment of recombinant coxsackievirus B3 injection. Contents of the invention [0003] The purpose of the present invention is to solve the shortcoming that there are relatively large errors in the evaluation method for the safety of recombinant coxsackie virus B3 injection in the prior art, and propose a safety method for recombinant coxsackie virus B3 injection evaluation method. [0004] In order to achieve the above object, the present invention adopts the following technical soluti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 蔡立刚
Owner WUHAN BOWEIDE BIOTECH CO LTD