Detection system and detection kit for PDGFRA gene mutation

A gene and system technology, applied in the field of detection of PDGFRA gene mutation, can solve the problems of high price, low cost, cumbersome accuracy, etc., achieve high sensitivity, simplify the experimental process, and reduce the false positive rate

Pending Publication Date: 2018-04-13
上海赛安生物医药科技股份有限公司
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Problems solved by technology

At present, the methods for PDGFRA gene mutation detection mainly include chip technology, probe primer technology, enzym

Method used

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  • Detection system and detection kit for PDGFRA gene mutation
  • Detection system and detection kit for PDGFRA gene mutation
  • Detection system and detection kit for PDGFRA gene mutation

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Embodiment Construction

[0034] The present invention is specifically described below through the examples, it is necessary to point out that the following examples are only used to further illustrate the present invention, and can not be interpreted as limiting the protection scope of the present invention, those skilled in the art can according to the above-mentioned present invention Contents Some non-essential improvements and adjustments are made to the present invention. In the following examples, unless otherwise specified, all reagents used are of analytical grade, and all reagents used can be obtained from commercial sources. For the experimental methods that do not indicate the specific conditions in the text, usually follow the conditions described in the book "Molecular Cloning Experiment Guide" published by Science Press, edited by J. Sambrook et al. in 2002, or according to the manufacturer's suggestion conditions of. Unless otherwise defined, all professional and scientific terms used ...

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Abstract

The invention relates to a detection system and a kit for PDGFRA gene mutation. The kit includes PCR detection solution A, PCR detection solution B and SYBR GreenI mixed solution; PCR detection solution A includes V516D for detecting PDGFRA gene exon 12 The upstream and downstream primer A and peptide nucleic acid A of the site, and the PCR detection solution B include upstream and downstream primer B and peptide nucleic acid B for detecting the D842V site of the 18th exon of the PDGFRA gene. The PDGFRA gene mutation detection system and the kit thereof of the present invention have high sensitivity and specificity, require less samples, and can quickly, conveniently and accurately perform PDGFRA gene mutation detection.

Description

technical field [0001] The invention relates to a method for detecting PDGFRA gene mutation and a detection product, belonging to the field of biotechnology. Background technique [0002] Studies have found that there are amplifications, deletions, and somatic mutations of PDGFRA in a variety of human cancers (gastrointestinal stromal tumors, glioblastomas, and malignant peripheral nerve sheath tumors). Studies have found that PDGFRA mutations not only It is related to the occurrence and development of human malignant tumors, and is also closely related to the staging, grading and prognosis of tumors. Therefore, the detection of PDGFRA mutations in different stages of tumor patients is of great significance for judging the prognosis of patients [0003] Exploring the mechanism of PDGFRA drug resistance and predicting the prognosis of patients has become a feasible way. It is now clear that tumor cells will release DNA into the circulation system, making it possible to analy...

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6886
CPCC12Q1/6858C12Q1/6886C12Q2600/156C12Q2600/118C12Q2525/107C12Q2563/107C12Q2531/113
Inventor 王明赵国玲
Owner 上海赛安生物医药科技股份有限公司
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