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Group of circular RNA (circRNA) markers for diagnosis of childhood acute lymphoblastic leukemia (ALL) and application of group of circRNA markers for diagnosis of childhood ALL

An acute lymphocyte and marker technology, applied in the field of circRNA markers for the diagnosis of acute lymphoblastic leukemia in children, can solve problems such as circRNA reports that have not yet been reported, and achieve the effects of broad clinical application prospects, high sensitivity and stable results.

Active Publication Date: 2018-04-20
NANJING CHILDRENS HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, circRNAs with early diagnostic value have been found in malignant tumors such as esophageal cancer, gastric cancer, and liver cancer, but there are no reports of related circRNAs in the field of children with ALL.

Method used

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  • Group of circular RNA (circRNA) markers for diagnosis of childhood acute lymphoblastic leukemia (ALL) and application of group of circRNA markers for diagnosis of childhood ALL
  • Group of circular RNA (circRNA) markers for diagnosis of childhood acute lymphoblastic leukemia (ALL) and application of group of circRNA markers for diagnosis of childhood ALL
  • Group of circular RNA (circRNA) markers for diagnosis of childhood acute lymphoblastic leukemia (ALL) and application of group of circRNA markers for diagnosis of childhood ALL

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Experimental program
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Embodiment 1

[0020] 1. Clinical sample collection

[0021] Bone marrow samples from 40 newly diagnosed children with ALL and 30 controls from normal children were collected from Children's Hospital Affiliated to Nanjing Medical University in 2016. All participants signed the informed consent.

[0022] 2. circRNA RT-PCR experiment

[0023] 2.1 RNA extraction and quantitative PCR

[0024] Total RNA was extracted from bone marrow samples using Trizol, and RNA was quantified using a visible light spectrophotometer.

[0025] 2.2 Primers

[0026] A group of circRNAs provided by the present invention include has_circ_0117909 and has_circ_0005720. They were all screened from the results of circRNA chip detection in children with ALL patients, using GAPDH as an internal reference gene (SEQ ID No: 7), and the primer sequences are shown in Table 1:

[0027] Table 1 Primer sequences used in RT-PCR

[0028]

[0029] 2.3 Reverse transcription reaction

[0030] The total RNA was reverse-transcr...

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Abstract

The invention discloses a group of circRNA markers for diagnosis of childhood ALL and application of the group of the circRNA markers for the diagnosis of childhood ALL. The markers comprise two circRNAs which are has_circ_0117909 as shown in SEQ ID No: 1 and has_circ_0005720 as shown in SEQ ID No: 2. The circRNA biomarkers for diagnosis of childhood ALL provided by the invention can be used forthe diagnosis of the childhood ALL, AUC of the circRNA biomarkers for distinguishing bone marrow of childhood ALL from normal control bone marrow can reach 0.962, the sensitivity reaches 90.9%, and the specificity reaches 95%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a group of circRNA markers for the diagnosis of acute lymphoblastic leukemia in children and applications thereof. Background technique [0002] Leukemia is a common malignant proliferative disease of the hematopoietic system. It is a malignant proliferative disease in which hematopoietic stem cells are blocked at a certain stage during the differentiation process. Acute lymphoblastic leukemia (ALL) in children is the most common malignant tumor in childhood, accounting for about 25-30% of malignant tumors in children under 15 years old. Worldwide, its annual incidence is about 3-4 / 100,000 population. There are more than 10,000 new cases in my country every year, and there is a trend of increasing year by year. Although with the continuous improvement of the diagnosis and treatment of children's ALL, the application of multi-drug combination therapy, supportive care and hematopoietic...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 方拥军薛瑶康美云王娅萍戎留成
Owner NANJING CHILDRENS HOSPITAL
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