A method for extracting synephrine from ripe navel orange peel

A technology of navel orange and peel, applied in the field of natural plant extraction

Active Publication Date: 2020-04-24
HEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most studies on synephrine are mainly extracted from Citrus aurantium, and there are few reports on extracting from mature pericarp.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting synephrine from ripe navel orange peel, comprising the following steps:

[0023] (1) Pulverization: Take the dried navel orange peel and pulverize it into particles with a particle size of 0.03 mm to 0.3 mm to obtain navel orange peel powder.

[0024] (2) The navel orange peel powder and distilled water are mixed according to the ratio of 1g: 10mL according to the weight to volume ratio, then add 0.6wt% pectin-cellulose compound enzyme of the weight of the navel orange peel powder weight, and enzymolyze it in a water bath at 55°C for 1h, Obtain the enzymatic hydrolysis mixture. Wherein, in the pectin-cellulose compound enzyme, the mass ratio of pectin and cellulase is 6:1, the enzyme activity of pectinase is 50000U / g, and the enzyme activity of cellulase is 60000U / g.

[0025] (3) The enzymolysis mixture is placed in an autoclave for ultra-high pressure deactivation treatment, the pressure is 300MPa, the time for deactivation treatment is 15min, ...

Embodiment 2

[0029] A method for extracting synephrine from ripe navel orange peel, comprising the following steps:

[0030] (1) Pulverization: Take the dried navel orange peel and pulverize it into particles with a particle size of 0.03 mm to 0.3 mm to obtain navel orange peel powder.

[0031] (2) The navel orange peel powder and distilled water are mixed according to the ratio of 1g: 15mL according to the weight volume ratio, then add 0.8wt% pectin-cellulose compound enzyme of the weight of the navel orange peel powder weight, and enzymolyze it in a water bath at 50°C for 2h, Obtain the enzymatic hydrolysis mixture. Wherein, in the pectin-cellulose compound enzyme, the mass ratio of pectin and cellulase is 5:1, the enzyme activity of pectinase is 50000U / g, and the enzyme activity of cellulase is 60000U / g.

[0032] (3) The enzymolysis mixture is placed in an autoclave for ultra-high pressure deactivation treatment, the pressure is 200MPa, the time for deactivation treatment is 25min, and...

Embodiment 3

[0036] A method for extracting synephrine from ripe navel orange peel, comprising the following steps:

[0037] (1) Pulverization: Take the dried navel orange peel and pulverize it into particles with a particle size of 0.03 mm to 0.3 mm to obtain navel orange peel powder.

[0038] (2) The navel orange peel powder and distilled water are mixed according to the ratio of 1g: 12mL according to the weight volume ratio, and then 0.7wt% pectin-cellulose compound enzyme of the weight of the navel orange peel powder weight is added, and the enzyme is hydrolyzed in a water bath at 60°C for 1h, Obtain the enzymatic hydrolysis mixture. Wherein, in the pectin-cellulose compound enzyme, the mass ratio of pectin and cellulase is 7:1, the enzyme activity of pectinase is 50000U / g, and the enzyme activity of cellulase is 60000U / g.

[0039](3) The enzymolysis mixture is placed in an autoclave for ultra-high pressure deactivation treatment, the pressure is 250MPa, the time for deactivation trea...

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Abstract

The invention discloses a method for extracting synephrine from ripe navel orange peel, which comprises the following steps: (1) crushing the dried navel orange peel and mixing it with distilled water, adding a compound enzyme for enzymolysis to obtain an enzymolysis mixture; 2) The enzymolysis mixture is subjected to ultra-high pressure deactivation treatment, and the pH is adjusted to 5.0-6.0, and then subcritical extraction is carried out under the protection of nitrogen, and the supernatant is collected by centrifugation after extraction; (3) The molecular cut-off is 5000u~ A 10000u ultrafiltration membrane is used to perform ultrafiltration separation on the supernatant obtained in step (2), and collect the concentrate; (4) mix and stir the concentrate obtained in step (3) with ethanol, and the resulting mixture is filtered under reduced pressure and frozen Dry to get synephrine. The method has the advantages of high extraction rate and extraction efficiency, mild treatment conditions, and environmental friendliness.

Description

technical field [0001] The invention belongs to the technical field of natural plant extraction, in particular to a method for extracting synephrine from ripe navel orange peel. Background technique [0002] Navel oranges are abundant in Fuchuan County, Hezhou City, Guangxi, and their peels are the main by-products in the process of eating and processing. According to the survey, there is less comprehensive utilization of tribute orange and navel orange peels in this area. At present, except for a small amount of citrus peels used for processing tangerine peels, there is basically no other use. Most of them are discarded as waste, which not only wastes precious resources, but also seriously pollute the environment. In fact, citrus peels contain many active ingredients such as synephrine and hesperidin, so they are raw materials with potential industrial production value. Synephrine is an alkaloid that constricts blood vessels, raises blood pressure, and strongly dilates tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C213/10C07C215/60
CPCC07C213/10C07C215/60
Inventor 蔡文段振华帅良
Owner HEZHOU UNIV
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