The invention discloses amino acid organic liquid fertilizer and a preparation method thereof. The amino acid organic liquid fertilizer is prepared from the following components in parts by weight: 90to 110 parts of poultry feather, 4 to 6 parts of glucose, 4 to 6 parts of biogas slurry, 15 to 20 parts of macro elements, 1 to 5 parts of trace elements and 0.8 to 1.5 parts of chelating agent. Thepreparation method comprises the following steps: adding the glucose into the biogas slurry, fermenting in a closed manner to obtain a fermented solution; then adding the poultry feather into the fermented solution, fermenting in a closed manner to prepare an amino acid solution, then adding the chelating agent, macro elements and trace elements into the amino acid solution to react, finally cooling to the room temperature, thus obtaining the amino acid organic liquid fertilizer. The amino acid organic fertilizer is prepared by adopting microorganisms to ferment the poultry feather. By adopting the preparation method, no racemization effect is produced, the environment is not polluted, the production cost is low, the obtained amino acid is high in biological activity and low in content oftoxic and harmful articles; and meanwhile, the amino acid organic liquid fertilizer provided by the invention is easier to absorb by crops, so that the resource waste can be reduced.