Method for extracting concentrated S-adenosylmethionine

A technology of adenosylmethionine and feed liquid, which is applied in the field of biopharmaceuticals, can solve the problems of high toxicity, poor specificity, and strong irritation of trichloroacetic acid, and achieve the elimination of milk making and demulsification processes, and the separation process is simple and reliable. The effect of service life

Active Publication Date: 2012-08-01
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, trichloroacetic acid is highly toxic and irritating, and the follow-up treatment is complicated and costly
There are patent literature reports in Japan that activated carbon is used to adsorb impurities in the fermentation broth, but its specificity is poor and the yield is low
Therefore, traditional separation and purification methods such as solvent extraction, precipitation, and adsorption have problems such as complex processes, the use of a large amount of organic solvents, poor specificity, low efficiency, and high energy consumption.

Method used

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  • Method for extracting concentrated S-adenosylmethionine
  • Method for extracting concentrated S-adenosylmethionine
  • Method for extracting concentrated S-adenosylmethionine

Examples

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Embodiment 1

[0045] Figure 4 It is a schematic diagram of the process of extracting and concentrating SAM using synchronous analytical support liquid membrane separation technology, which is now passed Figure 4 The specific technical process of this embodiment is described.

[0046] Step 1: The biological fermentation liquid of SAM in the feed-liquid phase container 1 is the feed-liquid phase, wherein the concentration of SAM is 10 mM, the total volume of the feed-liquid phase is 800 mL, and the pH value of the feed-liquid phase is adjusted to 3.0 with phosphoric acid. Start the constant flow pump 4 to circulate the feed liquid phase through the tube side of the hollow fiber membrane module 7, the flow rate of the constant flow pump is 400mL / min, and the pressure of the pressure gauge 12 is controlled by the ball valve 10 to 9psi.

[0047] Step 2: The extractant is AOT / DEHPA / TOPO, the molar ratio is 5:30:2, n-octanol is used as the solvent, and the volume of the liquid film phase is 800...

Embodiment 2

[0053] Step 1: Use hydrochloric acid to adjust the pH value of the liquid phase of the feed to 3.5, the flow rate of the constant flow pump is 100mL / min, the pressure of the pressure gauge 12 is 5psi, and other conditions are the same as in Example 1.

[0054] Step 2: The extractant AOT / DEHPA / TOPO molar ratio is 2:10:1, with n-butanol as solvent, weak electrolyte solution is 1.0mM sodium acetate solution, acetic acid to adjust pH to 2.0, add 0.5mM sodium chloride . The flow rate of the constant flow pump is 200mL / min, the pressure of the pressure gauge 13 is 3psi, the pressure gauge 14 is about 2psi, and other conditions are the same as in Example 1.

[0055] Step 3: the weak electrolyte solution in the secondary analytical phase is 0.5 mM sodium acetate solution, the pH is adjusted to 1.0 with hydrochloric acid, and 0.5 mM sodium chloride is added. The flow rate of the constant flow pump is 100mL / min, the pressure of the pressure gauge 15 is 5psi, and other conditions are th...

Embodiment 3

[0060] Step 1: Phosphoric acid is used to adjust the pH value of the liquid phase of the material to 3.0, the flow rate of the constant flow pump is 800mL / min, the pressure of the pressure gauge 12 is 10psi, and other conditions are the same as in Example 1.

[0061] Step 2: The molar ratio of extractant AOT / DEHPA / TOPO is 6:50:3, dedecyl alcohol is used as solvent, the weak electrolyte solution is 1.0 mM sodium phosphate solution, the pH is adjusted to 2.5 with hydrochloric acid, and 3.0 mM sodium chloride is added. The flow rate of the constant flow pump is 1000mL / min, the pressure of the pressure gauge 13 is 5psi, the pressure of the pressure gauge 14 is about 4psi, and other conditions are the same as in Example 1.

[0062] Step 3: The weak electrolyte solution in the secondary analytical phase is 1.0 mM sodium phosphate solution, the pH is adjusted to 1.5 with hydrochloric acid, and 2.0 mM sodium chloride is added. The flow rate of the constant flow pump is 800mL / min, the ...

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Abstract

The invention belongs to the field of biopharmaceuticals, and relates to a method for extracting concentrated S-adenosylmethionine. The method uses a new synchronous analytic supported liquid membrane separation technology; in a supported liquid membrane separation system, a feed phase, a first order analytic phase and a second order analytic phase are aqueous solution phases, and are connected with a water-immiscible organic liquid membrane to form an immiscible multiphase system, and all phases are connected into a whole by an interfacial chemical reaction; and an organic solution containing an extraction agent is adsorbed in a microporous support body of a hydrophobic hollow fiber membrane by a supported liquid membrane under the action of intermolecular force and capillaries, and a substance to be separated is transferred to the analytic phase through the liquid membrane phase from the feed phase by utilizing an interface coordination chemistry reaction at both sides of the liquid membrane, a facilitated transport action generated in the liquid membrane, and an analytic reaction between the liquid membrane phase and analytic phase interfaces. According to the method, emulsifying and demulsifying working procedures are eliminated, so that the separation process is simpler, more reliable and more practical, the cost is greatly reduced, and the large-scale production is easy to implement.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a new method for extracting and concentrating S-adenosylmethionine from a biological fermentation liquid by using a synchronous analytical support liquid membrane separation technology. Background technique [0002] S-adenosylmethionine, also known as S-adenosylmethionine, English name S-Adenosyl-methionine (SAM) is the active form of methionine. SAM is a chiral substance, only (S, S)-SAM, that is, (-)-SAM has biological activity, its chemical structure is shown in figure 1 . [0003] SAM is an important physiologically active substance, which participates in various biochemical reactions in organisms, and exhibits extensive and diverse therapeutic effects. It has good curative effects on arthritis, depression, liver dysfunction, etc. , cardiovascular disease and anti-aging health care medicines have good clinical application prospects, and the demand for...

Claims

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Application Information

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IPC IPC(8): C07H19/167C07H1/06
Inventor 刘卫万江陵唐宇翔周小顺杨祥良
Owner HUAZHONG UNIV OF SCI & TECH
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