Method for producing amino acid, oligopeptide, calcium lactate and chitin by treating shrimp shell waste through solid-state fermentation of streptomyces and application of amino acid, oligopeptide, calcium lactate and chitin

A solid-state fermentation and waste technology, applied in the field of bioengineering, can solve the problems of ineffective recycling, high equipment requirements, high cost, etc., and achieve the effect of increasing utilization value, reducing processing procedures, and improving utilization rate

Active Publication Date: 2022-05-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with amino acids and oligopeptides, the price of large-molecular-weight shrimp shell proteins is not high (about 6,000 yuan / ton). It needs further acid hydrolysis, alkali hydrolysis or enzymatic hydrolysis to become oligopeptides or amino acids to increase its value. High requirements on equipment, cumbersome process and high cost
Shrimp shells contain a lot of minerals, and existing technologies often cannot effectively recycle them, resulting in waste
At present, there is no report on the simultaneous recovery of amino acids, polypeptides, calcium lactate, and chitin from shrimp shell waste.

Method used

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  • Method for producing amino acid, oligopeptide, calcium lactate and chitin by treating shrimp shell waste through solid-state fermentation of streptomyces and application of amino acid, oligopeptide, calcium lactate and chitin
  • Method for producing amino acid, oligopeptide, calcium lactate and chitin by treating shrimp shell waste through solid-state fermentation of streptomyces and application of amino acid, oligopeptide, calcium lactate and chitin
  • Method for producing amino acid, oligopeptide, calcium lactate and chitin by treating shrimp shell waste through solid-state fermentation of streptomyces and application of amino acid, oligopeptide, calcium lactate and chitin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Determination of various components of shrimp shell waste

[0058] After the shrimp shell waste was dried at 65°C, the shrimp shell waste powder was obtained, which was stored at -20°C for future use. The methods for determining protein, minerals, chitin and moisture in shrimp shell waste are as follows:

[0059] (1) Protein: Because chitin contains nitrogen, in order to eliminate interference, the shrimp shell powder was first treated with 1M NaOH solution at 90°C for 3 hours, and the protein concentration of the supernatant was determined by the Kjeldahl method (GB 5009.5-2016). The protein content in the shrimp shell was measured to be 54.52%;

[0060] (2) Minerals: use 1M hydrochloric acid solution to treat the shrimp shell powder at 40°C for 6 hours to remove minerals, measure the weight loss after drying, and the mineral content in the shrimp shells can be measured, which is 20.10%;

[0061] (3) Chitin: firstly treat the shrimp shell powder with 1M...

Embodiment 2

[0063] Embodiment 2: process shrimp shell by Streptomyces solid state fermentation

[0064] Streptomyces sp. SCUT-1 described in Chinese patent application CN201910491700.8, a Streptomyces strain and its application in degrading feathers, was used as the fermentation strain. Streptomyces sp. SCUT-1, with the preservation number GDMCC No:60612, was preserved in the Guangdong Provincial Microbial Culture Collection Center on March 20, 2019, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City.

[0065] Streptomyces sp. SCUT-1 was inoculated on Gaoshi No. 1 solid medium, pH = 7.2, and cultured at 37°C for 7 days to obtain a Streptomyces sp. SCUT-1 spore plate.

[0066] Streptomyces sp. SCUT-1 spores were inoculated in LB medium, pH = 7.2, and cultured at 40°C for 18 hours to obtain a seed fermentation broth with OD = 7-10 at a wavelength of 660nm;

[0067] Shrimp shell waste was sieved using a standard sieve to obtain shrimp shell waste with different particle size...

Embodiment 3

[0074] Example 3: Separating amino acids and oligopeptides from production and analyzing their components

[0075] Streptomyces sp. SCUT-1 was inoculated on Gaoshi No. 1 solid medium, pH = 7.2, and cultured at 37°C for 7 days to obtain a Streptomyces sp. SCUT-1 spore plate.

[0076] Streptomyces sp. SCUT-1 spores were inoculated in LB medium, pH = 7.2, and cultured at 40°C for 18 hours to obtain a seed fermentation broth with OD = 9 at a wavelength of 660nm;

[0077] 25g of shrimp shell waste powder with a particle size of 2.0-5.0mm, 0.3g / L of potassium dihydrogen phosphate, 0.4g / L of dipotassium hydrogen phosphate, 0.5g / L of sodium chloride and 100mL of water were prepared as a fermentation medium; That is: the solid-liquid ratio of shrimp shell waste powder to the water used in the fermentation medium is 1:4, sterilized by moist heat at 121°C for 20 minutes, and after cooling to room temperature, add the seed fermentation liquid with OD=9 at 660nm wavelength, in which the se...

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Abstract

The invention discloses a method for producing amino acid, oligopeptide, calcium lactate and chitin by treating shrimp shell waste through streptomyces solid state fermentation and application of the method, and belongs to the technical field of biological engineering. The invention relates to a method for producing amino acid, oligopeptide, calcium lactate and chitin by treating shrimp shell waste through streptomycete solid state fermentation, which comprises the following steps: inoculating streptomycete seed fermentation liquor into a fermentation culture medium containing the shrimp shell waste to obtain fermentation liquor, and separating to obtain first supernate and first precipitate; and preparing an amino acid product, membrane separation raffinate, an oligopeptide product, chitin and calcium lactate. According to the method, a large amount of amino acid is produced from shrimp shell waste for the first time, the recovery rate reaches 61.55%, the free amino acid concentration reaches 82.5 g / L, the amino acid productivity reaches 16.5 g / (L * d), the content of essential amino acid reaches 58.52%, and the shrimp shell waste is an excellent medicine and health care product raw material, a food nutrition additive and a feed additive.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for producing amino acids, oligopeptides, calcium lactate and chitin by using streptomyces solid-state fermentation to process shrimp shell waste and its application. Background technique [0002] China is one of the largest shrimp farming countries in the world, with a production of 2.07 million tons in 2020 (IMARC-Group, Research and Markets, 2021). Guangdong Province is a major aquatic province in China. According to statistics, the total production of aquatic products in Guangdong will reach 8.8032 million tons in 2020, and the output of shrimp alone will exceed 1 million tons, most of which will be processed into shrimps for commercial use. But the fleshy part of shrimp is less than 45% of total weight, and the rest is treated as solid organic waste. Incredibly fast growth. On the other hand, shrimp shell waste is a good biological resource, suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C12P21/00C12P19/04C12P3/00A23K20/142A23K20/147A23K20/24A23K20/163A61K31/195A61K31/198A61K35/612C12R1/465
CPCC12P13/04C12P21/00C12P19/04C12P3/00A23K20/142A23K20/147A23K20/24A23K20/163A61K35/612A61K31/198A61K31/195
Inventor 罗晓春陆德林倪静涛李志伟陆雯珺张明舒
Owner SOUTH CHINA UNIV OF TECH
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