A method for enzymatically preparing levodopa

Abstract

The invention discloses a method for preparing levodopa by enzymatic method. Insert 3 to 10% of the inoculum into the fermentation medium for fermentation and culture, centrifuge after fermentation, and collect the bacterial cells; add tyrosine and bacterial cells to the buffer solution, at 18 to 30°C, pH5. Under the condition of 0-6.0, the enzymatic reaction is carried out to convert tyrosine into levodopa. In the conversion process, the present invention adopts static cell conversion technology, preferably intermittent weak ventilation technology, which improves the catalytic efficiency of the enzyme, the concentration of levodopa can reach 27g / L, and the molar conversion rate of tyrosine can reach more than 99%, and Mild conditions, high enantiomer selectivity, no racemization, more suitable for industrial production, with significant economic benefits and industrial application value.

Description

technical field

[0001] The invention relates to a method for preparing levodopa, in particular to a method for enzymatically preparing levodopa, which belongs to the technical field of microorganisms. Background technique

[0002] Levodopa (L-DOPA), whose chemical name is 3,4-dihydroxyphenylalanine, is an important intermediate product in the biochemical metabolic pathway from L-tyrosine to catechol or melanin. Dopamine, a derivative of L-DOPA, is an important neurotransmitter. Since dopamine cannot enter the brain tissue through the blood-brain barrier, Parkinson's disease cannot be treated by supplementing dopamine, and L-DOPA can pass through the blood-brain barrier. And decarboxylation in the brain tissue to form dopamine, so that the content of dopamine in the brain tissue increases to achieve the purpose of treatment. Birkmayer in 1961 with levodopa treatment achieved significant results. L-DOPA and compound levodopa (such as Madopar) have become the most effective d...