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Nuttallia olivacea larva large-scale breeding method

A technology of blood clam and Zicai, which is applied in the field of large-scale seed breeding of Zicai blood clam, can solve the problems of inability to produce seedlings for multiplication and release and artificial breeding, insufficient bait, low survival rate, etc., and achieve large-scale Significant benefit, stable seed yield, and improved survival rate

Active Publication Date: 2018-05-01
日照市海洋与渔业研究所
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Zicai blood clam is a kind of bivalve mollusk that lives in the low tide zone and subtidal zone of the coastal intertidal zone. Due to its delicious and unique taste, thin shell and high meat yield, consumers have demanded Zicai blood clam in recent years. In addition, the discharge of coastal pollutants has destroyed its living environment and seriously affected the reproductive survival rate of Zicai blood clams; therefore, the survival rate of Zicai blood clams has been declining. trend
At present, the artificial seedling breeding technology of Violet blood clam has been studied, but the supply of single-celled algae is extremely unstable, the pollution of protozoa in the algae cultivation pool is frequent, and the bait is seriously insufficient after the larvae lay down. In addition, due to the immature cultivation method of larvae, there are The survival rate is low, the average survival rate is only 30%, and the number of seedlings is extremely unstable. It is impossible to produce large quantities of high-quality seedlings to meet the needs of multiplication and release and artificial breeding.
Compared with the existing artificial breeding technology of Zicai blood clam, the large-scale breeding method of Zicai blood clam seed has the advantages of stable supply of single-cell algae, high hatching rate of fertilized eggs, good larvae quality, and 30-50% increase in larval survival rate , It can stably produce 100 million high-quality commercial seedlings with a shell length ≥ 2mm, and has met the requirements for large-scale seed production. However, the large-scale breeding of Zicai blood clam seedlings has been blank in the aquatic industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Preparation of bait:

[0034] Step 1-1, start cultivating single-cell algae first-class algae species in December in winter, the species are Chrysoflagellates, 3011, Pavlova, microdiatoms, chlorella, Qingdao flatweed, Chaetoceros, etc., in containers It is a 10-liter or 20-liter small-mouth transparent glass bottle. The water for cultivation is seawater that has been filtered through precipitation→sand filter→protein foam separator→1000 mesh bag, and then boiled at 100℃ and cooled to 15-25℃. The water for diatoms is 15.0°C, the water for Chrysoflagellates, 3011, Pavlova, microdiatoms, Chlorella, and Sclerophytum Qingdao is 20°C, and the water for Chaetoceros is 25.0°C. The algae seed room is air-conditioned for 24 hours to maintain the temperature at 24°C;

[0035] Step 1-2, the preparation of secondary algae species is carried out in February in spring. The containers are 75 liters and 500 liters of small white plastic buckets. -6 g / ml bleach solution (available c...

Embodiment 2

[0059] 1) Preparation of bait:

[0060] Step 1-1, start cultivating single-cell algae first-class algae species in December in winter, the species are Chrysoflagellates, 3011, Pavlova, microdiatoms, chlorella, Qingdao flatweed, Chaetoceros, etc., in containers It is a small transparent glass bottle of 10 liters and 20 liters. The water for cultivation is the seawater that is filtered through sedimentation→sand filter→protein foam separator→1000 mesh bag, then boiled at 100℃ and cooled to 15℃~25℃, such as 15.5°C, 16°C, 16.5°C, 17°C, 17.5°C, 18°C, 19°C, 20°C, 20.5°C, 21°C, 21.5°C, 22°C, 22.5°C, 23°C, 23.5°C, 24°C, 24.5°C Etc. The algae seed room is air-conditioned for 24 hours to keep the temperature at 20-26°C, such as 20.2°C, 20.5°C, 21°C, 21.5°C, 22°C, 22.5°C, 23°C, 23.5°C, 24°C, 24.5°C, 25°C, etc. ;

[0061]Step 1-2, the preparation of secondary algae species is carried out in February in spring. The containers are 75 liters and 500 liters of small white plastic buckets. ...

Embodiment 3

[0085] 1) Four-level bait preparation:

[0086] Step 1-1, from December to January in winter or 3 to 4 months before brood spawning, start to cultivate single-celled algae first-class algae species, such as Isochrysis or Chrysoflagellates, 3011, Pavlova , small diatoms, chlorella, Qingdao flatweed, Chaetoceros, the water for cultivation is filtered through sedimentation→sand filter→protein foam separator→1000 mesh bag, and then boiled at 100℃ and cooled to 18℃~ Seawater at 20°C;

[0087] Step 1-2, the preparation of secondary algae species is carried out from February to March in spring. The container is a small white plastic bucket of 100 liters to 400 liters. Bleach solution with a content of 5% to 8% 500×10 -6 After 24-48 hours of disinfection with g / ml, use 30×10 -6 g / ml sodium thiosulfate for neutralization, use after no residual chlorine is determined by starch potassium iodide solution;

[0088] Step 1-3, the production of three-stage algae liquid is carried out fro...

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Abstract

The invention discloses a nuttallia olivacea larva large-scale breeding method, relates to the technical field of aquaculture, and particularly belongs to the nuttallia olivacea larva large-scale breeding method. The method is characterized by comprising the following steps of four-grade bait preparation, wherein a first-stage unicellular alga species starts to be cultivated from December to January in winter or 3-4 months before spawning of parent shellfish, second-stage alga species is prepared from February to March in spring, a three-stage alga solution is produced from March to May in spring, and large-scale unicellular bait is produced in a pond from April to August in spring and summer; parent shellfish collection and spawning; fertilized egg hatching; larva breeding. The method hasthe advantages that the method is not limited by natural conditions, the yield is high, and large-scale benefits are remarkable.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for large-scale seed breeding of purple blood clams. Background technique [0002] Zicai blood clam is a kind of bivalve mollusk that lives in the low tide zone and subtidal zone of the near-shore intertidal zone. Due to its delicious and unique taste, thin shell and high meat yield, consumers have demanded Zicai blood clam in recent years. In addition, the discharge of coastal pollutants has destroyed its living environment and seriously affected the reproductive survival rate of Zicai blood clams; therefore, the survival number of Zicai blood clams has been declining. trend. At present, the artificial seedling breeding technology of Violet blood clam has been studied, but the supply of single-celled algae is extremely unstable, the algae cultivation pool is frequently polluted by protozoa, and the bait is seriously insufficient after the larvae lay down. In additi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54A01K61/17A23K10/30A23K50/80
CPCA01K61/17A01K61/54A23K10/30A23K50/80Y02A40/81
Inventor 王雪梅崔浩孙玉忠郭建军田璐
Owner 日照市海洋与渔业研究所
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