Method for quickly purifying wheat thermo-photo sensitive genic male sterile line
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A technology of male sterile line and photosensitive sterile line, which is applied in the field of rapid purification of wheat thermophotosensitive male sterile line, can solve the problems of short service life of sterile line and long time of purification, and achieve stable purification effect
Pending Publication Date: 2018-05-08
FOOD CROPS RES INST YUNNAN ACAD OF AGRI SCI
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Problems solved by technology
There are two major problems in the purification of sterile lines by this method. One is that the purification period is longer; the other is that it is only for phenotype purification, and the purified sterile line has a short service life, and the above purification work must be repeated
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Embodiment 1
[0021] A method for rapidly purifying a temperature-sensitive male sterile line of wheat, comprising the steps of:
[0022] In the first year, typical strains with "seed jumping" phenomenon (abnormal fruiting) were selected in the winter-sowed wheat sterile line K78S breeding field, and typical sterile characteristic strains of K78S were screened and bagged;
[0023] In summer, the harvested bagged plant seeds are planted into rows. When the wheat grows and develops to the heading stage, the stamens are removed before self-pollination, and corn pollen is given after the stigmas are scattered. After 24 hours of pollination, the ears are cut and cultivated indoors.
[0024] The most suitable amount of ear culture solution is to submerge the wheat stalks by 2-3cm with the culture solution, which can ensure that the stalks can absorb enough nutrients, and minimize the erosion of the stalks by the culture solution, prolonging the length of the stems, Leaf function period, while als...
Embodiment 2
[0028] Late sowing in winter of 2007, wheat sterile line K78S breeding field, screening 102 typical sterile strains of K78S, bagging,
[0029] In the summer of 2007, 321 double haploid lines were obtained by using the technique of wheat haploid induction by hybridization of wheat and corn.
[0030] It was sown in winter in 2008, identified by stages, and 126 lines with similar fertility to K78S and completely homozygous genes were screened out.
[0031] In the summer of 2008, multiplication was carried out, and 126 original stocks of K78S lines with stable fertility and homozygous genes were obtained.
[0032] Using this method only takes 2 years to obtain 126 completely homozygous double haploid wheat sterile lines, and the purified lines are still being used for seed production and propagation.
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Abstract
The invention discloses a method for quickly purifying wheat thermo-photo sensitive genic male sterile line, comprising the following steps: late sowing in winter, screening a typical K78S sterilize characteristic strain in the breeding field of the wheat sterile line K78S, performing bagging treatment on the typical K78S sterilize characteristic strain; harvesting the seeds of the bagged strain next summer, obtaining the double haploid lines of the wheat thermo-photo sensitive genic male sterile line by using a technology of inducing the wheat haploid by hybridization of wheat and corn; sowing in next winter by two stages, identifying by microscopic examination by stages, screening a line with similar fertility with K78S and with completely homozygous gene; performing expanding propagation in next summer to obtain a breeder's seed with stable fertility and homozygous gene of K78S. Compared with the traditional purifying method, the time can be shortened to half at least, the double haploid lines of the wheat sterile line with homozygous gene can be obtained within two years. According to the invention, purifying can be performed from genotype, the purifying effect is stable, the method can be used permanently and continuous purifying is not needed after the purifying, identifying and isolation and propagation are performed onece.
Description
technical field [0001] The invention relates to the technical field of wheat breeding, in particular to a method for rapidly purifying thermophotosensitive male sterile lines of wheat. Background technique [0002] The world's annual wheat planting area is about 3.3 billion mu, which is an important ration crop for human beings. The utilization of wheat heterosis is considered to be one of the effective ways to further improve the yield level, stress resistance and yield stability, and to enrich the genetic basis of wheat varieties. The two-line hybrid wheat technology system (hereinafter referred to as "two-line hybrid wheat") established on the basis of wheat environment-sensitive GMMS lines (mainly including three types: temperature-sensitive type, light-temperature-sensitive type, and temperature-sensitive type) is The new method of utilizing wheat heterosis created by our country has become the mainstream technology in the research and application of hybrid wheat in ou...
Claims
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