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Application of Gelnectin alkaloids as Biomarkers of Toxic Honey

A technology of poisonous honey and honey, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., and can solve problems such as alkaloid types and chemical structures that are not clarified

Inactive Publication Date: 2020-07-07
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been recognized that gecko alkaloids are present in the toxic honey of gecko, the type and chemical structure of the alkaloid has not been elucidated

Method used

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  • Application of Gelnectin alkaloids as Biomarkers of Toxic Honey
  • Application of Gelnectin alkaloids as Biomarkers of Toxic Honey
  • Application of Gelnectin alkaloids as Biomarkers of Toxic Honey

Examples

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Embodiment 1

[0038] Test case:

[0039] 1. Test materials: nectar from ginkgo, poisonous honey from ginkgo and common honey samples (duck foot wood honey, black tallow honey, acacia honey, wattle honey, rape honey, lychee honey, jujube flower honey, longan honey and hundred flower honey) ; Among them, the nectar and poisonous honey of Gelacia were obtained in December 2016 in the area where honey poisoning occurred in Shaoguan, Guangdong, and a large group of Gelkins was found on the hillside 3km away from the site of the honey poisoning incident. December is the peak flowering period of the ginkgo. At this time, a small amount of ginkgo nectar can be absorbed with a glass capillary. A Chinese bee farmer was found 1.5km down the mountain, and a sample of its honey was collected as a positive sample of hook kiss honey. Through the identification of the pollen morphology of the collected honey samples, it was confirmed that the pollen of the honey was contained in the honey, so it was infer...

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Abstract

The invention relates to the field of food safety, and specifically discloses gelsemium alkaloids (gelsemine and / or gelsenicine) as biomarkers for toxic honey and an application thereof in identification of the toxic honey. Researches find that the gelsemium alkaloids are especially suitable for recognition and identification of the toxic honey and early diagnosis of honey-poisoning people. On thebasis, furthermore, provided is a UHPLC-Q Exactive plus or LC-MS / MS detection method for the two gelsemium alkaloids, and pretreatment conditions and detection parameters of honey samples are optimized. The biomarkers and the detection method for the toxic honey provided by the invention are of great realistic significance in protection of life and health of honey consumers and maintaining of healthy development of honey consumption industries.

Description

technical field [0001] The present invention relates to the field of food safety, in particular, relates to the use of gelzis alkaloids as biomarkers of poisonous honey and their application in identifying poisonous honey. Background technique [0002] Honey is a natural sweet substance that is fully brewed by bees from the nectar, secretion or honeydew of plants collected by bees, combined with their own secretions. Due to the high nutritional value of honey, it is favored by consumers, and its sales volume is also on the rise year by year. However, in recent years, there have been many mass incidents of poisoning and even death caused by eating "poisonous honey" in many areas in southern my country. For example, in 2016, 13 people were poisoned in Guangzhou, and 1 child died; in 2016, 4 people were poisoned in Zhaoping, Guangxi. 1 death, 2 deaths in Zhong County, Chongqing in 2016, 1 death in Weixi, Yunnan in 2016, 11 poisonings and 4 deaths in Enshi, Hubei in 2015, 19 poi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 杨术鹏李熠周金慧张金振金玥王鹏赵文
Owner BEE RES INST CHINESE ACAD OF AGRI SCI