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A kind of application and preparation method of pyrrole-2-carboxylic acid
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A technology of carboxylic acid and pyrrole, which is applied in the field of application and preparation of pyrrole-2-carboxylic acid, and can solve problems such as crop diseases
Active Publication Date: 2020-08-18
SHENZHEN UNIV
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[0003] The present invention provides a kind of application and preparation method of pyrrole-2-carboxylic acid, aiming at solving the problem that plant fungi cause diseases to crops
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[0012] The invention provides an application and preparation method of pyrrole-2-carboxylic acid. Pyrrole-2-carboxylic acid has the activity of resisting plant pathogens, and can be applied to resist plant pathogens, thereby inhibiting plant pathogens in crops or ecological environment. Then solve the harm of plant diseases to crops and the ecological environment.
[0013] The present invention also provides a kind of preparation method of pyrrole-2-carboxylic acid, mainly comprises the following steps:
[0014] Step 1. Inoculate the Flavobacterium into the culture medium, culture and ferment for at least 96 hours at a temperature of 26-28° C. and a shaker speed of 160-200 rpm to obtain a fermentation broth;
[0015] Flavobacterium Arenibacter sp.6A1 was preserved in the China Center for Type Culture Collection (CCTCC for short) on May 15, 2017, at Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province. Serial number M2017262, designated 6A1.
[0016] Speci...
Embodiment
[0033] 1. Strain fermentation:
[0034] The cryopreserved Flavobacterium CCTCCM2017262 was inoculated into the fermentation medium and cultured for 96 hours. Wherein, the rotation speed of the shaker of the culture medium is 180 rpm, and the temperature is 28°C. The medium is 2216E medium (the formula of 2216E medium is: peptone 5g / L, yeast extract 1g / L, ferric phosphate 0.01g / L, sea salt 20g / L), and the medium is autoclaved at 121°C for 20 minutes.
[0035] 2. Separation of pyrrole-2-carboxylic acid:
[0036] Ethyl acetate extraction: Use 1mol / L hydrochloric acid to adjust the pH of the fermented medium to 3-4, extract with ethyl acetate, rotate the extract to dryness at a temperature lower than 50°C, and repeat the extraction process three times to obtain ethyl acetate Ester layer extract 2g.
[0037] The ethyl acetate layer extract was dissolved in 2mL of methanol, and carried out Sephadex LH20 (column specification: diameter=3.5cm, column length=100cm) column chromatogr...
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Abstract
The invention provides an application and preparation method of pyrrole-2-carboxylic acid, which relates to the technical field of compound preparation. Among them, pyrrole-2-carboxylic acid is used for resisting plant pathogenic fungi. Pyrrole-2-carboxylic acid has anti-plant pathogenic activity, and it can be applied to resist plant pathogens, thereby inhibiting plant pathogens in crops or ecological environment, and then solving the harm of plant diseases to crops and ecological environment.
Description
technical field [0001] The invention belongs to the technical field of compound preparation, in particular to an application and preparation method of pyrrole-2-carboxylic acid. Background technique [0002] Phytopathogenic fungi refer to fungi that can parasitize on plants and cause plant diseases, and are an important source of infection for plant diseases. Usually, several or even dozens of fungal diseases can be found on a crop, which seriously harms the ecological environment and causes economic losses to the production of crops. Contents of the invention [0003] The invention provides an application and preparation method of pyrrole-2-carboxylic acid, aiming at solving the problem that plant fungi cause diseases to crops. [0004] The invention provides an application of pyrrole-2-carboxylic acid, which is used to resist plant pathogenic fungi. [0005] The invention provides a kind of preparation method of pyrrole-2-carboxylic acid, comprising: [0006] inoculat...
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