Novel method for efficiently separating diatom

Abstract

The invention discloses a novel method for efficiently separating diatom, which mainly comprises the step of culture solution preparation and the step of separation and purification of diatom, whereinseparation and purification of diatom comprises the following steps: a water sample which contains diatom is collected; a screening tulle is used for filtering the water sample; the water sample is sterilized; the diatom undergoes enrichment culture; the density of the diatom is calculated; the diatom is separated; a test tube which contains the diatom is placed under an appropriate condition forculture; a newly separated diatom species is microscopically examined, and if no other organisms and foreign bacteria exist, then separation is successful. The advantages of the novel method are as follows: consumed time is short, operation is simple, the problem that target diatom is polluted by recipient bacteria and the problem that working efficiency is affected by the probability of successexisting in the process of transferring are prevented, and the working efficiency of separating and purifying diatom can be greatly increased on the whole. The separation effect is good, the density of obtained diatom is high, and the novel method is applicable to the separation and purification of diatom from natural water, aquaculture water, recipient bacterium pollution or other foreign alga pollution, and therefore has a good application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new method for efficiently separating diatoms, especially to the rapid separation and purification of diatoms. Background technique [0002] The cultivation and application of microalgae first require algae species. Algae species are first separated from the mixed biota in natural waters by certain methods, obtained monoculture, and preserved for cultivation. Separating and purifying microalgae is an indispensable technology for its scientific research. Diatoms are one of the main producers of marine organic matter and one of the primary productive forces of the ocean. At the same time, diatoms are also direct or indirect bait for marine animals and their larvae. Diatoms account for 54% of the annual food of Chinese hairy shrimp in natural sea areas. Young fish such as herring and sardines also use diatoms as their main food. It can be seen that the production of marine economi...

AI Technical Summary

Problems solved by technology

The amount of agar added to the solid medium should be used flexibly according to the quality of the agar. If the medium is too hard, it will affect the slow release of nutrients, and the water will be easily lost, which is not conducive to the growth of algae colonies....