A method for preparing fluorescent carbon dots using chloroplast as carbon source

A technology of fluorescent carbon dots and chloroplasts, applied in the field of chemical applications, can solve the problems of unsuitable fluorine-containing solutions, high impedance of glass electrodes, easy damage, etc., and achieve the effect of simple process and rapid preparation

Active Publication Date: 2021-07-30
HUNAN UNIV OF SCI & TECH
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Problems solved by technology

[0003] In the early days, people used the electrochemical method to measure the pH value. However, due to the high impedance of the traditional glass electrode, it is easily damaged, and it is not suitable for the measurement of pH in fluorine-containing solutions, and there is a "sodium error" in a highly alkaline environment. Many problems encountered in pH measurement

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  • A method for preparing fluorescent carbon dots using chloroplast as carbon source
  • A method for preparing fluorescent carbon dots using chloroplast as carbon source
  • A method for preparing fluorescent carbon dots using chloroplast as carbon source

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Embodiment 1

[0034] Chloroplast extraction: Take spinach as an example: select fresh spinach leaves, wash them with water, wash them with secondary water for 2-3 times, and remove the larger veins in spinach leaves after drying. Accurately weigh 10 g of spinach leaves, chop them into pieces, put them into a mortar, add 20 mL of 0.35 mol / L NaCl solution for grinding. The homogenate after grinding was filtered into a 100mL beaker with six layers of gauze, and the filter residue was discarded. The filtered filtrate was transferred to a 5 mL centrifuge tube, centrifuged at 1000 r / min for 10 min, and the precipitate was discarded. Transfer the supernatant to a 5mL centrifuge tube and continue to centrifuge at 3000r / min for 20min, then discard the supernatant and take the lower layer of sediment, wash with 0.35mol / L NaCl solution for 2-3 times, then add 10mL 0.35mol / L NaCl The solution will suspend the precipitate. A suspension containing chloroplasts is obtained.

[0035] Preparation of fluo...

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Abstract

The invention belongs to the technical field of chemical application, and in particular relates to a fluorescent carbon dot solution synthesized by a hydrothermal method using chloroplast as a carbon source and used for pH detection. The method for detecting the pH value of the solution includes the following steps: using chloroplast as the carbon source, water as the dispersion liquid, and synthesizing the fluorescent carbon dot solution under the heating condition of the hydrothermal reaction kettle. Obtain the carbon dot solution with fluorescence characteristics; After the fluorescent carbon dot solution reacts with the solution of different pH, there is a good linear relationship between the fluorescence intensity value and the pH value of the fluorescent carbon dot solution F = 395.44‑10.36x, where I F is the fluorescence intensity value, x is the pH value, and the detectable pH range of the solution is 1.83-10.38. The invention has a simple process, can quickly prepare fluorescent carbon dots, and can accurately measure the pH of the solution.

Description

technical field [0001] The invention belongs to the technical field of chemical application, and specifically relates to a fluorescent carbon dot solution synthesized by a hydrothermal method using chloroplast as a carbon source; the pH of the solution can be detected by utilizing the different fluorescent intensity values ​​of the fluorescent carbon dot solution reacting with solutions of different pH . Background technique [0002] Fluorescent carbon dots are a new type of nanomaterial with a diameter of less than 10nm, which has become a research hotspot in the carbon nano family because of its low toxicity, stable chemical properties, excellent water solubility, photobleaching resistance, and easy functionalization. These excellent properties of fluorescent carbon dots make it have broad application prospects in the fields of biomarkers, environmental analysis, photoelectric sensing and so on. At present, it is still difficult to achieve uniform synthesis of carbon dots...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/15C09K11/65G01N21/64B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C01P2004/64C01P2006/60C09K11/65G01N21/643
Inventor 陈述温傲立冉勇丁琳成奋民陈建龙云飞曾荣今
Owner HUNAN UNIV OF SCI & TECH
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