A method for preparing fluorescent carbon dots using chloroplast as carbon source
A technology of fluorescent carbon dots and chloroplasts, applied in the field of chemical applications, can solve the problems of unsuitable fluorine-containing solutions, high impedance of glass electrodes, easy damage, etc., and achieve the effect of simple process and rapid preparation
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[0034] Chloroplast extraction: Take spinach as an example: select fresh spinach leaves, wash them with water, wash them with secondary water for 2-3 times, and remove the larger veins in spinach leaves after drying. Accurately weigh 10 g of spinach leaves, chop them into pieces, put them into a mortar, add 20 mL of 0.35 mol / L NaCl solution for grinding. The homogenate after grinding was filtered into a 100mL beaker with six layers of gauze, and the filter residue was discarded. The filtered filtrate was transferred to a 5 mL centrifuge tube, centrifuged at 1000 r / min for 10 min, and the precipitate was discarded. Transfer the supernatant to a 5mL centrifuge tube and continue to centrifuge at 3000r / min for 20min, then discard the supernatant and take the lower layer of sediment, wash with 0.35mol / L NaCl solution for 2-3 times, then add 10mL 0.35mol / L NaCl The solution will suspend the precipitate. A suspension containing chloroplasts is obtained.
[0035] Preparation of fluo...
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