A method for accurately determining the content of chitosan oligosaccharides by resonance Rayleigh scattering method
A resonance Rayleigh scattering and accurate measurement technology, applied in the field of macromolecular detection, can solve the problems of enhanced resonance scattering intensity and cumbersome operation techniques
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[0072] A method for accurately measuring chitosan content by resonance Rayleigh scattering method, comprising the steps of:
[0073] 1) Draw the standard curve of △I and different concentrations of chitosan oligosaccharides
[0074] Add 1.0mL of B-R buffer solution with a pH of 4.9, a certain concentration gradient of chitosan oligosaccharide standard solution, 1.0mL of sodium lauryl sulfate solution with a concentration of 0.086mol / L, and 1.0mL of a concentration of 1.0× 10 -4 mol / L ruby red solution, use distilled water to make up the volume, shake well, place it at room temperature for 10min, and then measure it on the F-2500 fluorescence spectrophotometer with λ ex =λ em Carry out synchronous scanning, record the resonance scattering intensity I of each detection system containing chitosan oligosaccharide at λ=470nm, and the resonance scattering intensity I of the reagent blank at λ=470nm 0 , and calculate △I=I-I 0 , Establish the standard curve C of ΔI and chitosan ...
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