Method for increasing sexual propagation success rate of Daphne aurantiaca Diels

Abstract

The invention belongs to the technical field of biological propagation, and discloses a method for increasing the sexual propagation success rate of Daphne aurantiaca Diels. According to the method, the floral traits are measured and analyzed, the pollen activity is measured through an MTT staining method, the stigma receptivity is measured through a benzidine-hydrogen peroxide method, and the morphology of a stigma and pollen is observed through a scanning electron microscope. The method shows that the floral structure of the Daphne aurantiaca Diels is special, is tubulous and comprises anthers of two wheels, a pistil and a stamen are separated from each other; the pollen of a flower in bloom has the activity, the stigma has the receptivity, under the scanning electron microscope, the structures of the stigma and the pollen are not abnormal, and some pollen falls onto the stigma; pollinators for the Daphne aurantiaca Diels are phalaenae which visit the flower at night, and the flowervisiting frequency is low; P/O and hand pollination experiments show that a breeding system of the Daphne aurantiaca Diels is outcrossed; and the fruit-set ratio of the Daphne aurantiaca Diels is verylow, the fruit-set ratio of the natural pollination is 1.4%, and the fruit-set ratio of hand cross pollination is 23.3%.

Description

technical field [0001] The invention belongs to the technical field of biological reproduction, and in particular relates to a method for improving the success rate of sexual reproduction of Daphne neroli. Background technique [0002] Low fruit set is a fatal obstacle in the sexual reproduction of seed plants, limiting the continuation and spread of species. The fruit setting rate of plants is closely related to pollination, and pollination is a necessary stage for the reproduction of seed plants (Wu Peiwen et al., 2016). Usually successful pollination needs to meet the following conditions: 1. Both pollen and stigma have normal structure and function; 2. Have enough pollinators and sufficient frequency of flower visits to ensure the placement of a sufficient amount of pollen on the stigma; 3. Stigma and pollen They can successfully recognize each other (Wu Peiwen et al., 2016). Studies have found that woody plants are more likely to show low fruit-setting rates (because ...

AI Technical Summary

Problems solved by technology

This feature limits the further development and utilization of this genus, but the reason is still unclear, and there are few related studies, especially in my country, no one has carried out

[0003] In summary, the problems in the prior art are: Daphne genus has important economic and ornamental value, its application and market prospects are broad, and the low fruit setting rate under natural conditions also means that its natural reproductive ability is low, For example, Daphne neroli, the object of the present invention, has a natural fruit setting rate of only 1.4%, which severely limits the propagation and spread of the species, and also seriously affects the further development and utilization of the species. So far, no one has studied the related problems, and there is no feasible solution. proposal

The reason for the low fruit setting rate of Daphne genus under natural conditions is not yet clear, and there are few studies

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