Application of seneciphylline in preparation of antitumor medicines

An anti-tumor drug, the technology of Guangfeiling, applied in the field of biomedicine, can solve the problem of unclear mechanism of anti-tumor action

Inactive Publication Date: 2018-06-01
LONGYAN UNIV
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Problems solved by technology

[0005] Seneciphylline (SENE) is an alkaloid derived from the root of Compositae [Gynura segetum (Lour) Merr]. It is said that Senecion Feiling has an anti-tumor effect, but its anti-tumor mechanism is still unclear and needs further research

Method used

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  • Application of seneciphylline in preparation of antitumor medicines
  • Application of seneciphylline in preparation of antitumor medicines
  • Application of seneciphylline in preparation of antitumor medicines

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. Unless otherwise specified, the reagents, equipment and methods used in the present invention are commercially available reagents, equipment and routinely used methods in this technical field.

[0027] 1. Sources of reagents and materials:

[0028] Human cervical cancer HeLa cell line was purchased from ATCC;

[0029] DMEM high-glucose medium and fetal bovine serum were purchased from Hyclone Company;

[0030] Senecion Feiling (SENE) was purchased from Chengdu Desite Biotechnology Co., Ltd. and dissolved in dimethyl sulfoxide (DMSO);

[0031] MTT kit, RIPA lysate, and ECL chemiluminescent reagents were purchased from Biyuntian Biotechnology Co., Ltd.;

[0032] Opti-MEM medium, 3000 and Lysotraker Red were purchased from Invitrogen;

[0033] G418 was purchased from Gibco;

[0034] DMSO, trehalose (Trehalose, Tre), 3-methyladenine ...

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Abstract

The invention discloses an application of seneciphylline in preparation of antitumor medicines. The seneciphylline can inhibit HeLa cell proliferation and induce HeLa cell autophagy in a dose dependent and a time dependent manner. In addition, the seneciphylline inducing the HeLa cell autophagy is depended on activation of MEK/ERK1/2 pathway. The invention provides a novel treatment medicine of treating human cervical cancer.

Description

technical field [0001] The present invention relates to the field of biomedicine, and more specifically relates to the application of a plant active substance Senecio Feiling in inducing tumor cell autophagy and inhibiting tumor cell proliferation. Background technique [0002] Worldwide, cervical cancer is a serious threat to the health and life of women of reproductive age. It is the second most common malignant tumor in women and one of the main causes of women's morbidity and death. In recent years, its incidence has shown a younger trend. . At present, chemotherapy is one of the main treatment methods for cervical cancer patients. However, chemotherapy resistance makes cervical cancer treatment ineffective and relapses clinically. How to overcome chemotherapy resistance of cervical cancer is a major challenge for its treatment. [0003] In recent years, the anti-tumor research of traditional Chinese medicine active substances has attracted more and more attention from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/407A61P35/00
Inventor 马景蕃张燕叶甘萍韦启良邱龙新
Owner LONGYAN UNIV
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