Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of microRNA-106b to preparation of medicines for preventing and treating liver injury and products for diagnosing liver injury

A technology for liver injury and drug-induced liver injury, which is applied in the field of medicine and can solve the problem of unidentified microRNA

Active Publication Date: 2018-06-01
ZHEJIANG UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, although more and more microRNA molecules have been discovered as biomarkers, determinants and therapeutic targets of human diseases, microRNAs that play a key role in the adaptive response to liver injury have not been identified, and their functions are crucial to the development of human diseases. Still a challenge for researchers in the field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of microRNA-106b to preparation of medicines for preventing and treating liver injury and products for diagnosing liver injury
  • Application of microRNA-106b to preparation of medicines for preventing and treating liver injury and products for diagnosing liver injury
  • Application of microRNA-106b to preparation of medicines for preventing and treating liver injury and products for diagnosing liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Analysis of serum exosome microRNA expression profile in mouse toosendanin liver injury response model

[0057] According to the previous literature of the inventors (Lu X, Ji C, Tong W, Lian X, Wu Y, Fan X, Gao Y. Integrated analysis of microRNA and mRNA expression profiles highlights the complex and dynamic behavior of toosendanin-induced liver injury in mice.SciRep . 2016; 6:34225.doi:10.1038 / srep34225), constructing a mouse model of adaptive response to toosendanin liver injury; this literature study found that mice have adaptive phenomenon to the liver injury caused by toosendanin, and can well Simulate the occurrence and development of adaptive response to clinical drug liver injury, that is, biochemical evidence of liver injury occurs after 9 days of toosendanin administration, and the pathology shows necrosis of liver cells. The drug dose is not changed, and the drug dose is continued to 21 days, and the biochemical indicators recover The phenomenon of...

Embodiment 2

[0071] Example 2 Effects of miR-106b and anti-miR-106b on proliferation of damaged hepatocytes in vitro

[0072] Mouse embryonic liver cells (BNL CL.2) in the logarithmic growth phase were inoculated in 96-well plates, and placed at 37°C, 5% CO 2 After culturing in the incubator for 24 hours, the culture solution was aspirated and washed twice with PBS, and each well was added with 100 nmol miR-106b-5p agomir (microR-106b-5p agonist, that is, microR-106b-5p high expression group), agomir control (microR-106b-5p agonist control, basically no homology with other microRNA), miR-106b-5p antagomir (microR-106b-5p antagonist, that is, antagonize microR-106b-5p group) and antagomir control (microR-106b-5p group) -106b-5p antagonist control, basically no homology with other microRNA) of toosendanin culture solution 100 μL (containing 0.1 μM toosendanin solution prepared in 10% FBSDMEM), with 0.1 μM toosendanin solution as a control, to establish The in vitro liver injury model was cu...

Embodiment 3

[0075] Example 3 Effects of miR-106b and anti-miR-106b on mouse liver injury model

[0076] Male BALB / c mice were divided into 6 groups according to body weight: normal saline control group, toosendanin group, miR-106b-5pantagomir control group, antagomir control control group, miR-106b-5p agomir group, agomir control control group, n=5 or 6 (indicating the number of mice in each group). The mice in the antagomir group were injected with antagomir 100nmol / time in the tail vein, the mice in the agomir group were injected with agomir 10nmol / time in the tail vein, and the mice in the normal saline control group and the toosendanin group were respectively injected with an equal volume of normal saline every 3 days. The normal saline group was given CMC-Na by intragastric administration, and the other groups were given 80 mg / kg of toosendanin. During this period, the changes in the body weight of the mice were observed, and blood was taken from the tail on the 3rd and 11th days, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of microRNA-106b to preparation of medicines for preventing and treating liver injury and products for diagnosing liver injury. MicroRNA-106b, particularly microRNA0106b-5p, is firstly discovered through experiments, plays a key effect in the adaptive reaction of the liver injury, and the microRNA-106b can serve as a diagnosis marker for diagnosing and predicting liver injury reversion and also can serve as a medicine and a new target point for preventing and treating liver injury.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to the application of microRNA-106b in the preparation of drugs for preventing and treating liver damage and products for diagnosing liver damage. Background technique [0002] The liver is an important organ for the synthesis, biotransformation and detoxification of human substances, and it is usually the first and most important target organ for toxic side effects. Primary liver cancer, hepatitis, cirrhosis, fatty liver and drug-induced liver injury have become the most common liver diseases, which seriously plague developed countries such as Europe and the United States and developing countries such as China. So far, there is still a lack of effective prevention and treatment methods. [0003] The latest 2015 edition of my country's "Guidelines for the Diagnosis and Treatment of Drug-Induced Liver Injury" points out that "restorative tissue repair" is an intrinsic determinant of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7105A61P1/16
CPCA61K31/7105
Inventor 范骁辉陆晓燕
Owner ZHEJIANG UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com