Composition and preparation method thereof
A composition and solution technology, applied in the field of biomedical engineering, can solve the problems of limited curative effect and cannot prevent disease progression, etc., and achieve the effects of convenient acquisition, inhibition of inflammation, and repair of damaged tissue
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[0043] Such as figure 1 Shown, the present invention also provides a kind of preparation method of composition, comprises the steps:
[0044] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the mesoderm, a type of cell with multidirectional differentiation potential, which exist in human bone marrow, adipose tissue, umbilical cord of newborns, placenta and other tissues. It has the potential to differentiate into cartilage, fat and myoblasts, and also has the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiat...
Embodiment 1
[0055] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the umbilical cord of newborns. Mesenchymal stem cells not only have the potential to differentiate into bone, cartilage, fat, and myoblasts, but also have the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiation potential. The samples were stripped of Wharton's jelly in a biological safety cabinet, and in the culture bottle, 1g of Wharton's jelly was added to each bottle, and the incubator was CO 2 an incubator, and the mesenchymal stem cells are primary...
Embodiment 2
[0068] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the mesoderm, a type of cell with multidirectional differentiation potential, which exist in human bone marrow, adipose tissue, umbilical cord of newborns, placenta and other tissues. It has the potential to differentiate into cartilage, fat and myoblasts, and also has the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiation potential. The samples were stripped of Wharton's jelly in a biological safety cabinet, and in the culture bottle, 1g of Wharton's j...
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