Dengue virus vaccine

A dengue virus and vaccine technology, applied in the field of vaccines, can solve problems such as unclear immune mechanism

Inactive Publication Date: 2018-06-15
重庆卓诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the immune mechanism based on NS1 protein is still unclear, and most of them believe that humoral immunity plays a major role

Method used

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Embodiment Construction

[0019] Embodiments of the technical solution of the present invention will be described in detail below. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and therefore are only examples, rather than limiting the protection scope of the present invention.

[0020] 1. Construction of Dengue Virus Vaccine

[0021] 1. All adenoviral vectors were purchased from GeneChem;

[0022] 2. NS1cDNA was chemically synthesized according to the sequence of dengue type 2 virus Hainan strain recorded in GeneBank (NC_002020.1, whose sequence is shown in SEQ NO.1);

[0023] 3. When synthesizing the NS1 gene, Xba I site, Kozak signal and tissue plasminogen activator (TAP) sequence are added to the 5' end, and 2 terminators and Xba I site are added to the 3' end, To facilitate follow-up experimental operations; details are as follows: (1) total viral RNA extraction; (2) RT-PCR amplification of gene cDNA; (3) PCR product agarose gel ...

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Abstract

The invention provides a dengue virus vaccine. The invention starts from the currently rarely reported T-cell immunity of the dengue virus, a simian adenovirus carrier is used to construct the vaccineto break through the limitations of the human adenovirus carrier in the practical application, an Ad carrier vaccine C9-NS1 for the dengue virus II is successfully constructed, the carrier clones a PCR (Polymerase Chain Reaction) product rapidly through the clone technology, the double enzyme digestion operations of a plasmid and a target fragment are avoided, and the carrier is relatively convenient and efficient. A histidine tag is arranged at the end C, expressing the fusion protein, of the carrier, so that the affinity chromatography and purification of the recombinant protein is facilitated, and the protein of the histidine tag is relatively small and barely produces effects on the activity and the function of the expression protein. The immunologic function of the vaccine from the protein NS 1 of the dengue virus is evaluated comprehensively from multiple aspects, and a foundation is laid for the further research and development of the efficient spectrum dengue gene vaccine.

Description

technical field [0001] The invention relates to a vaccine, in particular to a dengue virus vaccine. Background technique [0002] Dengue virus (Dengue virus) is a small flavivirus, which belongs to the genus Flavivirus, and is the pathogen of a mosquito-borne acute infectious disease, leading to diseases such as dengue fever, dengue fever and dengue shock. It is usually transmitted by Aedes aegypti and Aedes albopictus mosquitoes, which bite people during the day. Dengue virus can cause a range of clinical symptoms, including life-threatening hemorrhagic shock syndrome and, less commonly, acute hepatitis with hepatic failure and encephalopathy. Infection with dengue virus can range from sudden fever, severe muscle pain, bone and joint pain, to extensive bleeding and rapid shock. [0003] Dengue virus is an enveloped single-strand, positive-strand RNA virus, including 4 different serotypes. The dengue virus genome is 10-11kb long, encoding 3 structural proteins (C, PrM, E) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K39/39A61K39/395A61P31/14C12N15/861C07K16/10C07K14/18
CPCA61K39/12A61K39/39A61K39/395A61K2039/5256A61K2039/55566C07K14/005C07K16/1081C12N15/86C12N2710/10343C12N2770/24122C12N2770/24134Y02A50/30
Inventor 程希汀刘剑书李晶
Owner 重庆卓诺生物技术有限公司
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