Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cultivated eggplant SmHQT gene core fragment, RNAi expression vector and application thereof

A core fragment and expression vector technology, applied in the field of genetic engineering, can solve the problems of ambiguity and complex synthesis routes, and achieve the effect of reducing the degree of browning and reducing the content of chlorogenic acid

Inactive Publication Date: 2018-06-15
ZHENGZHOU UNIV
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chlorogenic acid is synthesized from phenylalanine in the cytoplasm through a series of enzymatic reactions, but the synthesis pathway is very complex, and the key enzymes that control the synthesis of chlorogenic acid in eggplant are not clear in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cultivated eggplant SmHQT gene core fragment, RNAi expression vector and application thereof
  • Cultivated eggplant SmHQT gene core fragment, RNAi expression vector and application thereof
  • Cultivated eggplant SmHQT gene core fragment, RNAi expression vector and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The present invention will be described in detail below in conjunction with the accompanying drawings. For experimental methods that do not indicate specific conditions, usually follow the conventional conditions or the conditions suggested by the manufacturer.

[0031] The cultivated eggplant variety used in the examples of the present invention is "Solanum melongena L.cv. Sanyueqie". The pMD19-T vector is the product of TaKaRa company, pHBLD vector, pBin19 vector, Escherichia coli DH5α, Escherichia coli "helper" bacteria (referred to as helper) containing the motile plasmid pRK2013 and Agrobacterium tumefaciens LBA4404 are common materials in this field; RNA extraction reagents Cassettes, Restriction Enzymes, Premix Taq TM (TaKaRaTaq TM Version 2.0), MaxDNA Polymerase is the product of TaKaRa Company.

[0032] 1. Cloning of the core fragment of the cultivated eggplant SmHQT gene

[0033] According to the SmHQT gene sequence (KT259042.1), the specific primers Sm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cultivated eggplant SmHQT gene core fragment, an RNAi expression vector containing the same, and construction and application of the cultivated eggplant SmHQT gene core fragment. The cultivated eggplant SmHQT gene core fragment is shown in SEQ ID NO.3. According to the cultivated eggplant SmHQT gene core fragment, a cultivated eggplant variety with reduced chlorogenic acid synthesis through silent SmHQT gene, the purpose of reducing browning of pulp of cultivated eggplant, and a new cultivated variety has a good market prospect and economic value.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to the cultivation of the eggplant SmHQT gene, the RNAi vector constructed by using the gene and its application, and also relates to the construction method of the RNAi vector. Background technique [0002] Cultivated eggplant (Solanum melongena L.) occupies an important position in the vegetable market in my country. As a vegetable with outstanding economic benefits, the color and nutrient content of the cultivated eggplant are the key factors that determine its commodity value. Chlorogenic acid is oxidized to quinones in the presence of oxygen, which is the key factor leading to pulp browning. These factors seriously hinder the improvement of nutritional quality and post-processing of cultivated eggplant. [0003] As an important phenylpropanoid in Solanaceae plants, chlorogenic acid is widely accumulated in plants and has the highest content in fruits (the content of ch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/82C12N1/21A01H5/00A01H6/82C12R1/01
CPCC12N15/8218C12N15/8243C07K14/415C12N15/66
Inventor 张彦杰黄从辉黄进勇赵雪洁张桂兰张亚倩王文明
Owner ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com