A chitosan derivative with selective antibacterial properties and its preparation method
A chitosan derivative and selective technology, applied in medical preparations containing active ingredients, organic active ingredients, antibacterial drugs, etc., can solve problems such as infection and bacterial flora imbalance, achieve low hemolysis, and reduce pollution and energy consumption, the effect of mild reaction conditions
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Embodiment 1
[0039] Weigh 0.35g chitosan and dissolve in 35mL dilute acid solution to obtain chitosan aqueous solution. Dissolve arginine and leucine (50:1 molar ratio) in 15 mL of buffer solution and activate for 10 hours to obtain an amino acid activation solution with an amino acid concentration of 300 mg / mL. The amino acid activation solution was mixed with chitosan aqueous solution and reacted for 24 hours. After the reaction is finished, the product is purified to obtain the chitosan derivative with selective antibacterial properties.
[0040] According to the method requirements of GB / T 16886.5-2003 "Biological Evaluation of Medical Devices Part Five In Vitro Cytotoxicity Test", the chitosan derivatives prepared in this example were tested. The results show that the cell proliferation rate of human immortalized epidermal cells is 87% under the chitosan derivative solution prepared in this example at a concentration of 2 mg / mL.
[0041] The chitosan derivatives prepared in this exa...
Embodiment 2
[0045] Weigh 0.7g chitosan and dissolve in 35mL dilute acid solution to obtain chitosan aqueous solution. Lysine and isoleucine (0.1:1 molar ratio) were dissolved in 15 mL of buffer solution and activated for 6 hours to obtain an amino acid activation solution with an amino acid concentration of 600 mg / mL. The amino acid activation solution was mixed with chitosan aqueous solution and reacted for 24 hours. After the reaction is finished, the product is purified to obtain the chitosan derivative with selective antibacterial properties.
[0046] According to the method requirements of GB / T 16886.5-2003 "Biological Evaluation of Medical Devices Part Five In Vitro Cytotoxicity Test", the chitosan derivatives prepared in this example were tested. The results show that the cell proliferation rate of human immortalized epidermal cells is 93% under the chitosan derivative solution prepared in this example at a concentration of 2 mg / mL.
[0047] The chitosan derivatives prepared in t...
Embodiment 3
[0051] Weigh 0.035g chitosan and dissolve in 35mL dilute acid solution to obtain chitosan aqueous solution. Arginine oligomers and leucine (0.05:1 molar ratio) were dissolved in 15 mL of buffer solution and activated for 8 hours to obtain an amino acid activation solution with an amino acid concentration of 100 mg / mL. The amino acid activation solution was mixed with chitosan aqueous solution and reacted for 24 hours. After the reaction is finished, the product is purified to obtain the chitosan derivative with selective antibacterial properties.
[0052] According to the method requirements of GB / T 16886.5-2003 "Biological Evaluation of Medical Devices Part Five In Vitro Cytotoxicity Test", the chitosan derivatives prepared in this example were tested. The results show that the cell proliferation rate of human immortalized epidermal cells is 95% under the chitosan derivative solution prepared in this example at a concentration of 2 mg / mL.
[0053] The chitosan derivatives p...
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