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A kind of detection method and application of goat ctnnb1 gene insertion/deletion

A detection method and goat technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of lack of research on reproductive traits, lack of research, etc. low cost effect

Inactive Publication Date: 2020-02-21
NORTHWEST A & F UNIV
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Problems solved by technology

[0007] At present, there are many studies on CTNNB1 gene in human diseases or mice, but less studies in goats, especially on reproductive traits.

Method used

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  • A kind of detection method and application of goat ctnnb1 gene insertion/deletion
  • A kind of detection method and application of goat ctnnb1 gene insertion/deletion
  • A kind of detection method and application of goat ctnnb1 gene insertion/deletion

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Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.

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Abstract

The invention discloses a method for detecting goat CTNNB1 gene insertion / deletion and an application thereof. The method comprises the steps that a to-be-detected goat whole genome DNA is taken as atemplate, a goat CTNNB1 gene is augmented through a PLC technology, then agarose gel electrophoresis is performed, and it is identified whether or not the goat CTNNB1 gene has insertion / deletion sitepolymorphism. The correlation analysis results of a large sample (N)600) reveal that significant correlation exists between different types of CTNNB1 gene insertion / deletion and traits of the firstlambing of Shanbei white pile goats, and it is prompted that the different types of CTNNB1 gene insertion / deletion can be taken as a DNA mark improving the traits of the firstlambing of Shanbei white pile goats. The method for detecting goat CTNNB1 gene insertion / deletion can be used in mark-assisted selection breeding of reproduction traits of Chinese goats, and is conductive to quickly building goat genetic resource groups with a trait of high-yield goat number.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion (indel), in particular to the rapid and accurate detection of goat CTNNB1 gene NC_030829.1:g.41610-41636 26-bp insertion / deletion (indel) polymorphism methods and their applications. Background technique [0002] The goat industry occupies an important position in my country's national economy. Mutton is one of the main sources of meat. In many areas of our country, the market demand for mutton is very large, and the consumption has risen sharply, and the wool fabric is beautiful and elegant, warm and durable. Shaanxi white cashmere goat is one of the important goat breeds in Shaanxi Province. However, many factors can affect the goat industry including the northern Shaanxi white cashmere goat, such as genetic factors, reproductive performance, feeding management and so on. Among them, male reproductive physiology and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 潘传英张雪莲王珂闫海龙屈雷朱海鲸刘锦旺蓝贤勇
Owner NORTHWEST A & F UNIV
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