Mass spectrum flow cytometer detection kit capable of accurately typing tumor immune cell subsets

Abstract

The invention discloses a mass spectrum flow cytometer detection kit capable of accurately typing tumor immune cell subsets. The detection kit is composed of 42 antibodies having metal tags. In the invention, the kit, which has 42 channels and 42 antibodies, is developed, so that through mass spectrum flow cytometer detection, 42 antibodies can be detected simultaneously, thus achieving accurate typing of the tumor immune cell subsets. Tests prove that the kit can achieve systemic, comprehensive, dynamic and accurate typing to all immune cell subsets of tumor diseases without simple staining reference in each channel and calculation of compensation, thus saving samples.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a mass spectrometry flow detection kit for accurate typing of tumor immune cell subsets. Background technique [0002] The immune system plays an important role in the occurrence, development and prognosis of diseases. However, most of the current research methods focus on studying the functions and molecular mechanisms of several immune cell subsets in diseases. At the same time, due to the defects of technical means, the definition of various immune cell subsets in previous studies was not accurate enough, and the research results were too one-sided. For example, in traditional flow cytometry, the overlapping of fluorescent signals limits the increase in the number of fluorescent channels. The number of channels is small, and the emission spectra of the channels overlap seriously. It is necessary to set up a blank group, a negative control group, and a single-stained grou...

AI Technical Summary

Problems solved by technology

[0005] Therefore, in the study of the immune system in tumor diseases, there is a lack of systematic, comprehensive, dynamic, and precise descriptions of all immune cell subsets.

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