Mass spectrum flow cytometer detection kit capable of accurately typing tumor immune cell subsets

An immune cell and flow cytometric detection technology, applied in the field of cell biology, can solve the problem of lack of comprehensive, dynamic and accurate description of immune cell subpopulation system, achieve accurate typing and save samples

Inactive Publication Date: 2018-06-29
浙江普罗亭健康科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, in the study of the immune system in tumor diseases, there is a lack of systematic, comprehensive, dynamic, and precise descriptions of all immune cell subsets.

Method used

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  • Mass spectrum flow cytometer detection kit capable of accurately typing tumor immune cell subsets
  • Mass spectrum flow cytometer detection kit capable of accurately typing tumor immune cell subsets
  • Mass spectrum flow cytometer detection kit capable of accurately typing tumor immune cell subsets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 tests the staining effect of 42 kinds of antibodies

[0022] 1. Prepare fresh normal human peripheral blood and extract immune cells.

[0023] 2. Divide the immune cells into two groups, the control group and the experimental group, with about 3x10^6 cells in each group, resuspend them in PBS, adjust the volume to 1 mL, add 194Pt, and stain at room temperature for 2 minutes to distinguish the dead and alive cells.

[0024] 3. Add 2mL bovine serum albumin solution, centrifuge at 500g / 5min, remove the supernatant, add 50uL blocking solution (0.5uL human immunoglobulin solution, 0.5uL mouse immunoglobulin solution, 0.5uL rat immunoglobulin solution , 0.5uL hamster immunoglobulin solution, 48uL bovine serum albumin solution), and block on ice for 20min.

[0025] 4. Add 50uL bovine serum albumin solution to the control group as a blank control, add 50uL extracellular antibody mixture (0.5ul each of the 35 extracellular antibodies in Table 1, 32.5ul bovine serum...

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Abstract

The invention discloses a mass spectrum flow cytometer detection kit capable of accurately typing tumor immune cell subsets. The detection kit is composed of 42 antibodies having metal tags. In the invention, the kit, which has 42 channels and 42 antibodies, is developed, so that through mass spectrum flow cytometer detection, 42 antibodies can be detected simultaneously, thus achieving accurate typing of the tumor immune cell subsets. Tests prove that the kit can achieve systemic, comprehensive, dynamic and accurate typing to all immune cell subsets of tumor diseases without simple staining reference in each channel and calculation of compensation, thus saving samples.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a mass spectrometry flow detection kit for accurate typing of tumor immune cell subsets. Background technique [0002] The immune system plays an important role in the occurrence, development and prognosis of diseases. However, most of the current research methods focus on studying the functions and molecular mechanisms of several immune cell subsets in diseases. At the same time, due to the defects of technical means, the definition of various immune cell subsets in previous studies was not accurate enough, and the research results were too one-sided. For example, in traditional flow cytometry, the overlapping of fluorescent signals limits the increase in the number of fluorescent channels. The number of channels is small, and the emission spectra of the channels overlap seriously. It is necessary to set up a blank group, a negative control group, and a single-stained grou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N27/62
CPCG01N15/14G01N27/62G01N2015/1006
Inventor 石宏宇
Owner 浙江普罗亭健康科技有限公司
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