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Preparation method and application of antibacterial protein microcapsules

A technology of antibacterial protein and microcapsules, which is applied in the field of preparation of antibacterial protein microcapsules, can solve the problems that the stability and release performance of lysostaphin cannot be guaranteed, the release time and dose are difficult to control, and it is easy to induce immune rejection, etc., to achieve Realize the effects of fixed-point positioning therapy, avoiding immunogenicity and easy degradability, and simple and easy operation

Active Publication Date: 2018-07-06
赛因斯特(重庆)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique involves making tiny capsules that contain certain substances like proteins inside them. These small particles help it stay together well when mixed into solutions containing other ingredients. They also have specific properties such as being able to control their releasing rate over time without losing any active agents they were carrying. Overall this results in improved efficacy and reduced risk from side-effects caused by these chemicals.

Problems solved by technology

This patented describes different ways to make microspheres from certain materials called cathode ray tubes (CRT). These particles have unique characteristics such as high strength when they hit each other during use, long shelf life under physiologically relevant conditions, ability to store large amounts of liquid at room temperature, ease of production, low cost compared to current technologies like encasing them inside plastic shells made up mostly of glass fiber reinforced epoxy resin, making these objects easier to administer externally due to their strong biocide effects against harmful organisms found throughout human body's bloodstream systems.

Method used

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  • Preparation method and application of antibacterial protein microcapsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one: a kind of lysostaphin injection drop forming microcapsule preparation technology, such as figure 1 shown, including the following steps:

[0031] Weigh sodium alginate (molecular weight: 500,000-1,000,000), the mass concentration is: 10 grams, respectively put it into 100 ml of ultrapure water through a micropipette, and then add 0.001 mg of lysostaphin to obtain a lysostaphin mixture. With the flow rate of the micropipette as 6ml / s, the lysostaphylococcus mixed solution was dripped into the gel bath equipped with 20% zinc chloride solution, 30% calcium chloride solution and 50% barium chloride solution, and reacted for 6 hours. Centrifuge the reaction solution at 2000 rpm for 10 minutes in a centrifuge, take it out and wash it with ultrapure water or buffer solution to obtain microspheres with a size of 100 microns to 400 microns, namely to obtain lysostaphin microcapsules.

[0032] After testing, the dissolution rate of the lysostaphin microcapsules w...

Embodiment 2

[0033] Embodiment two: a kind of lysostaphin injection forming microcapsule preparation technology comprises the following steps:

[0034] Weigh carboxymethyl cellulose, the mass concentration is: 10 g, respectively put into 100 ml ultrapure water through a micropipette, and then add 0.001 mg of lysostaphin to obtain a lysostaphin mixture. With the flow rate of the micropipette as 6ml / s, the lysostaphylococcus mixed solution was dripped into the gel bath equipped with 20% zinc chloride solution, 30% calcium chloride solution and 50% barium chloride solution, and reacted for 6 hours. Centrifuge the reaction solution at 2000 rpm for 10 minutes in a centrifuge, take it out and wash it with ultrapure water or buffer solution to obtain microspheres with a size of 100 microns to 400 microns, namely to obtain lysostaphin microcapsules.

[0035] After testing, the dissolution rate of the lysostaphin microcapsules was: >86% in 15 minutes; RSD≤1.6%.

Embodiment 3

[0036] Embodiment three: a kind of lysostaphin injection forming microcapsule preparation technology comprises the following steps:

[0037] Weigh acrylic acid, the mass concentration is: 10 grams, respectively put into 100 ml ultrapure water through a micropipette, and then add 0.001 mg of lysostaphin to obtain a lysostaphin mixture. With the flow rate of the micropipette as 6ml / s, the lysostaphylococcus mixed solution was dripped into the gel bath equipped with 20% zinc chloride solution, 30% calcium chloride solution and 50% barium chloride solution, and reacted for 6 hours. Purification of lysostaphin microcapsules: Centrifuge the reaction liquid at 2000 rpm for 10 min in a centrifuge, take it out and wash it with ultrapure water or buffer solution to obtain microspheres of 100 microns to 400 microns, namely to obtain lysostaphin microcapsules.

[0038] After testing, the dissolution rate of the lysostaphin microcapsules was: >92% in 15 minutes; RSD≤1.5%.

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Abstract

The invention relates to a preparation method and application of antibacterial protein microcapsules. The preparation method of the antibacterial protein microcapsules comprises the following steps: dissolving a film forming material into ultrapure water through a trace rod to obtain a film forming material prefabrication solution; adding antibacterial protein into the film forming material prefabrication solution to obtain an antibacterial protein microcapsule prefabrication solution; dropping the antibacterial protein microcapsule prefabrication solution into a gel bath, formed by mixing a zinc chloride solution, a calcium chloride solution and a barium chloride solution, by utilizing the trace rod; after reacting for a period of time, thus obtaining an antibacterial protein microcapsulemixed solution; purifying the antibacterial protein microcapsule mixed solution to obtain the antibacterial protein microcapsules. According to the preparation method provided by the invention, glycyl-glycine endopeptidase is covered by selecting a microcapsule technology and controlled release and slow release functions of protein drugs can be realized, so that fixed-point and fixed-position treatment can be realized better and the preparation method has important meaning in the field of accurate medical treatment.

Description

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Claims

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Application Information

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Owner 赛因斯特(重庆)科技有限公司