Preparation method and application of a multifunctional ultrasensitive zn2+ two-photon detection fluorescent molecular probe

A fluorescent molecular probe and multifunctional technology, applied in the field of analytical chemistry, can solve the problems of inaccurate quantitative results, unfavorable naked eye detection, single detection method, etc., and achieve the effect of improving detection sensitivity, excellent specificity, and fast detection speed

Active Publication Date: 2021-03-02
武汉聚成医疗科技有限公司
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Problems solved by technology

This will lead to problems such as low detection limit of existing detection methods, inaccurate quantitative results, single detection method, unfavorable for naked eye detection, and limitations of imaging research.
These issues are very restrictive for the detection of trace zinc ions in cells

Method used

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  • Preparation method and application of a multifunctional ultrasensitive zn2+ two-photon detection fluorescent molecular probe
  • Preparation method and application of a multifunctional ultrasensitive zn2+ two-photon detection fluorescent molecular probe
  • Preparation method and application of a multifunctional ultrasensitive zn2+ two-photon detection fluorescent molecular probe

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Embodiment 1

[0050] Embodiment 1: Preparation of multifunctional ultrasensitive Zn 2+ two-photon detection probe

[0051] (1) Dissolve 2,4-dihydroxybenzaldehyde in ethanol at a concentration of 0.02-0.06g / mL, then add diethyl glutaconate at a concentration of 0.067g / mL, mix well and add anhydrous Piperidine 2-5 mL, refluxed for 24 hours, cooled, a yellow solid was precipitated, and the solid was recrystallized with absolute ethanol to obtain product 1;

[0052] (2) Dissolve product 1 in anhydrous pyridine at a concentration of 0.025g / mL, then add acetic anhydride at a concentration of 1mol / L, stir for 0.5h, add 40 times the mass of crushed ice, and stir for 10min to precipitate an off-white solid. The solid was eluted with acetonitrile-dichloromethane eluent with a volume ratio of 1:5, and rotary evaporated to obtain product 2;

[0053] (3) Dissolve product 2 in tetrahydrofuran at a concentration of 0.011 g / mL, then add 4% osmium tetroxide aqueous solution, stir for 0.5 h, then add sodiu...

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Abstract

The invention relates to a multifunctional ultrasensitive Zn 2+ Preparation method and application of fluorescent molecular probe for two-photon detection. First, product 1 is obtained by reacting 2,4-dihydroxybenzaldehyde, diethyl glutaconate and anhydrous piperidine in ethanol, and product 1 is reacted with acetic anhydride in anhydrous pyridine to obtain product 2, product 2 React with osmium tetroxide and sodium periodate in tetrahydrofuran to obtain product 3, react product 3 with anhydrous potassium carbonate in methanol to obtain product 4; finally, dissolve product 4 in ethanol, add dihydrazine pyridine and glacial acetic acid to reflux, Recrystallization and other steps, that is, the multifunctional ultrasensitive Zn 2+ Fluorescent molecular probes for two-photon detection. Suitable for Zn in biological samples 2+ It can be used in analytical chemistry, life organic analytical chemistry, disease pre-diagnosis and medical clinical testing and other related fields.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a multifunctional ultrasensitive Zn 2+ Preparation method and application of fluorescent molecular probe for two-photon detection. Background technique [0002] Zinc is one of the essential elements for living organisms. Its content in the human body is second only to iron, and it is the second most transition metal element. Zinc ions widely exist in the cells of the human body and play an important role in many physiological activities such as cell metabolism, gene expression, body immunity and nerve transmission. At present, the detection method of zinc ion is mainly based on fluorescent molecular probe detection method; although the reported methods have their own advantages, in general, their detection response ratio (10-60 times) needs to be improved, and the quantitative method and detection method still need to be improved. Some detection limitations imposed by the needl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D405/12C09K11/06G01N21/64
CPCC07D405/12C09K11/06C09K2211/1029C09K2211/1088G01N21/6486
Inventor 陈光姜翱李明顺周伟窦昆尤进茂
Owner 武汉聚成医疗科技有限公司
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