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A diagnosis and treatment reagent with sers/fluorescence dual-mode targeted tumor cell imaging and its preparation method

A technology for diagnosis and treatment of tumor cells, applied in the nano field, can solve the problems of low sensitivity, slow imaging speed, poor accuracy, etc., and achieve the effects of good chemical and optical stability, long fluorescence lifetime, and low fluorescence lifetime.

Active Publication Date: 2021-02-26
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently used imaging techniques all have a common disadvantage: low sensitivity and poor accuracy, especially the inability to clearly determine the boundaries of the tumor site, which is not conducive to tumor resection and treatment
However, the long integration time of SERS detection and the slow imaging speed have become a major obstacle for rapid detection technology in vivo.

Method used

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  • A diagnosis and treatment reagent with sers/fluorescence dual-mode targeted tumor cell imaging and its preparation method
  • A diagnosis and treatment reagent with sers/fluorescence dual-mode targeted tumor cell imaging and its preparation method
  • A diagnosis and treatment reagent with sers/fluorescence dual-mode targeted tumor cell imaging and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Dissolve AS1411 in KCl / K at pH 7.4 3 PO 4 Buffer solution, so that the concentration of AS1411 is 1 mg / mL, and then according to the condition that the molar ratio of silver nitrate and AS1411 is 6:1, add 5 μL silver nitrate solution to 20 μL AS1411 solution in a mixed ice bath (0-4 ℃) After shaking and reacting for 20 minutes, add 5 μL of sodium borohydride solution (the molar ratio of silver nitrate to sodium borohydride is 1:1) to the mixed solution, and react in a constant temperature shaker for 4 hours under dark conditions to obtain AS1411-protected silver Nanocluster (AS1411-Ag).

[0034] 300 mg of natural graphite powder was added to a mixed solution of 60 mL of sulfuric acid and 20 mL of nitric acid, ultrasonically reacted for 2 hours and then stirred at 100°C for 24 hours. After the reaction, cool to room temperature, dilute to 800mL with deionized water, adjust the pH value to 8 with sodium carbonate solution, then remove excess water and inorganic salts by...

Embodiment 2

[0042] Dissolve AS1411 in KCl / K at pH 7.4 3 PO 4 Buffer solution, so that the concentration of AS1411 is 1 mg / mL, and then according to the condition that the molar ratio of silver nitrate and AS1411 is 6:1, add 5 μL silver nitrate solution to 20 μL AS1411 solution in a mixed ice bath (0-4 ℃) After shaking and reacting for 20 minutes, add 5 μL of sodium borohydride solution (the molar ratio of silver nitrate to sodium borohydride is 1:1) to the mixed solution, and react in a constant temperature shaker for 4 hours under dark conditions to obtain AS1411-protected silver Nanocluster (AS1411-Ag).

[0043] 300 mg of natural graphite powder was added to a mixed solution of 60 mL of sulfuric acid and 20 mL of nitric acid, ultrasonically reacted for 2 hours and then stirred at 100°C for 24 hours. After the reaction, cool to room temperature, dilute to 800mL with deionized water, adjust the pH value to 8 with sodium carbonate solution, then remove excess water and inorganic salts by...

Embodiment 3

[0048] Dissolve AS1411 in KCl / K at pH 7.4 3 PO 4 Buffer solution, so that the concentration of AS1411 is 1 mg / mL, and then according to the condition that the molar ratio of silver nitrate and AS1411 is 6:1, add 5 μL silver nitrate solution to 20 μL AS1411 solution in a mixed ice bath (0-4 ℃) After shaking and reacting for 20 minutes, add 5 μL of sodium borohydride solution (the molar ratio of silver nitrate to sodium borohydride is 1:1) to the mixed solution, and react in a constant temperature shaker for 4 hours under dark conditions to obtain AS1411-protected silver Nanocluster (AS1411-Ag).

[0049] 400mg of natural graphite powder was added to a mixed solution of 75mL of sulfuric acid and 25mL of nitric acid, ultrasonically reacted for 2 hours and then stirred at 100°C for 24h. After the reaction, cool to room temperature, dilute to 1000mL with deionized water, adjust the pH value to 8 with sodium carbonate solution, then remove excess water and inorganic salts by rotary...

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Abstract

The invention discloses a diagnosis and treatment reagent with SERS / fluorescence dual-mode targeted tumor cell imaging and a preparation method thereof. The multifunctional anti-cancer aptamer AS1411 is used as a template to generate silver nanoclusters (AS1411-Ag) protected by AS1411. ), and then assemble it with modified positively charged graphene quantum dots (GOD) through electrostatic interaction, and finally adsorb anticancer drugs with aromatic ring structure through π-π interaction, and construct a SERS / fluorescence Therapeutic reagent GOD / AS1411‑Ag for bimodal targeted tumor cell imaging. The present invention designs and constructs a nanocomposite with diagnostic and therapeutic functions that integrates targeting (molecular level), dual-modal imaging diagnosis (SERS and fluorescence) and treatment for selected tumors, and solves the problem of single technology in different imaging technologies At the same time, it can realize the central goal of early targeted diagnosis and simultaneous in situ treatment of tumors.

Description

technical field [0001] The invention relates to nanotechnology, in particular to a diagnosis and treatment reagent with SERS / fluorescence dual-mode targeting tumor cell imaging and a preparation method thereof. Background technique [0002] Cancer has become the number one killer that endangers human health. Improving the accuracy of its early diagnosis is the key to overcoming tumors. If early screening and early diagnosis are carried out on the basis of simultaneous early treatment, the "integration of diagnosis and treatment" will be realized. It can greatly improve the cure rate, quality of life, survival period and survival rate of cancer patients. Therefore, it is of great significance to develop new multifunctional therapeutic agents (Theranostic Agent). [0003] The emergence of nanotechnology has brought new hope for the realization of the integration of cancer diagnosis and treatment. The application of nanotechnology to conquer cancer is to apply nanotechnology t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K31/704A61K31/519A61P35/00
CPCA61K31/519A61K31/704A61K49/00A61K49/0002A61K49/0067
Inventor 陈凤华杜俊平陈庆涛李爽爽赵豪爽方少明
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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