Method for simultaneously and rapidly determining various phenolic acid and tanshinone components in compound salvia milliorrhiza tablets

A rapid determination technology for compound Danshen tablets, which is applied in the directions of measuring devices, material separation, and material analysis, can solve the problems of simultaneous extraction of multiple components, difficulty in component quality analysis, and relatively difficult analysis, so as to improve quality The effect of control level, short preparation time and saving consumption

Inactive Publication Date: 2018-07-27
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since salvianolic acids are water-soluble components and tanshinones are fat-soluble components, the polarity of the two types of components is greatly different, and it is difficult to analyze simultaneously, especially the sample preparation is difficult to achieve the simultaneous extraction of multiple components, and the Panax notoginseng The influence of multiple components and excipients, etc., brings more difficulties to the quality analysis of multiple components
In addition, the longer the sample analysis time, the more organic solvents are consumed, which is not conducive to energy saving and environmental protection

Method used

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  • Method for simultaneously and rapidly determining various phenolic acid and tanshinone components in compound salvia milliorrhiza tablets
  • Method for simultaneously and rapidly determining various phenolic acid and tanshinone components in compound salvia milliorrhiza tablets

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Embodiment 1

[0053] (1) Preparation of reference substance solution: Accurately weigh the following reference substances in a 10mL volumetric flask: danshensu 0.90mg, protocatechualdehyde 0.42mg, caffeic acid 0.39mg, rosmarinic acid 1.20mg, shikonic acid 0.85mg, Add 0.60mg of salvianolic acid A, 5.10mg of salvianolic acid B, 1.00mg of dihydrotanshinone I, 1.95mg of cryptotanone, 1.73mg of tanshinone I and 2.60mg of tanshinone IIA, dissolve in 80% ethanol aqueous solution, and dilute to 10mL. Dubbed a mixed reference solution.

[0054] (2) Preparation of the test solution: Take 10 tablets of Compound Danshen Tablets, remove the coating, accurately weigh, and grind finely. Accurately weigh 0.500g of the powder and place it in a round bottom flask with a stopper, add 20mL of 80% ethanol aqueous solution precisely, set a stopper, and accurately weigh. Put it in a microwave-assisted extractor, microwave power 400W, temperature 70°C, extract for 1.5min, take it out, put it at room temperature, ...

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Abstract

The invention discloses a method for simultaneously and rapidly determining various phenolic acid and tanshinone components in compound salvia milliorrhiza tablets. The method comprises the followingsteps: mixing a product to be detected and an extracting solvent; after carrying out microwave assisted extraction, detecting by a high performance liquid chromatography; quantifying salvianic acid, protocatechuic aldehyde, caffeic acid, rosmarinic acid, lithospermic acid, salvianolic acid A, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA quantitation byadopting an external standard method. Under the condition that the compound salvia milliorrhiza tablets contain various complicated components, the method can be used for simultaneously determining 7phenolic acid components and 4 tanshinone components in the compound salvia milliorrhiza tablets; the quality analysis efficiency of the compound salvia milliorrhiza tablets and the quality control level of a product can be remarkably improved; the method has the advantages that a few of organic solvents are consumed, energy saving and environmental protection are saved, and a green and chemicaldevelopment concept is met.

Description

technical field [0001] The invention relates to the field of effective component analysis of compound traditional Chinese medicine preparations, in particular to a method for simultaneously and rapidly determining multiple phenolic acids and tanshinone components in compound danshen tablets. Background technique [0002] Compound Danshen Tablets is a traditional Chinese medicine commonly used in clinical treatment of cardiovascular and cerebrovascular diseases. Angina pectoris and other diseases. This product is composed of Danshen, Panax notoginseng and Borneol. In recent years, the incidence of cardiovascular and cerebrovascular diseases has been increasing day by day, and the clinical application of Compound Danshen Tablets has become more extensive, and the market sales volume has continued to increase. At present, there are many varieties and specifications of this preparation, and there are also many manufacturers. In order to ensure the curative effect and safety of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 房信胜王建华
Owner SHANDONG AGRICULTURAL UNIVERSITY
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