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A kind of rapid detection method of malachite green

A malachite green and detection method technology, applied in the preparation of test samples, electrochemical variables of materials, etc., can solve the problems of no substitute products, false positive prices, easy residues, etc., to reduce detection costs, easy to operate, high-efficiency The effect of sensitive detection

Active Publication Date: 2020-12-01
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, malachite green is also a bactericidal and insecticide with excellent performance. It has a good killing effect on common diseases of fish, shrimp and other aquatic products, such as saprolegniasis, gill mold and melon worm disease; but peacock Stone green is rapidly metabolized into colorless malachite green in fish. Colorless malachite green has higher toxicity and is easy to remain. At the same time, it has teratogenic and carcinogenic effects. It is a banned drug for aquaculture that is banned by most countries.
However, due to the low price and excellent effect of malachite green and there is no corresponding substitute product, it has been banned repeatedly.
Malachite green routine detection methods are mainly concentrated in two categories, one is physical and chemical detection methods, and the other is enzyme-linked immunoassay methods. These methods are either qualitative detection, or require large-scale instruments and professional operators, or have false positives. problematic and expensive because of the need to invent a simple, efficient, specific and inexpensive assay

Method used

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  • A kind of rapid detection method of malachite green
  • A kind of rapid detection method of malachite green
  • A kind of rapid detection method of malachite green

Examples

Experimental program
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Embodiment 1

[0035] The present embodiment provides a kind of malachite green rapid detection method, comprises the steps:

[0036] 1) Add 1 mL of sodium acetate buffer solution with a concentration of 0.1 mol / L and pH=4.5 to 2 μL of 0.1 mol / L sodium thiosulfate solution, and oscillate evenly to obtain a sensitizer mixture;

[0037] 2) After connecting the screen-printed electrode to the portable electrochemical workstation, immerse it in the sensitizer mixture prepared in step 1), select the square wave anodic stripping voltammetry, the activation potential window is 0.3V-0.7V, and the voltage increases Volume 4mV, amplitude 0.025V, frequency 15Hz, activate the screen printing electrode;

[0038] 3) After the activation is completed, add a malachite green solution with a concentration of 36ppb to the sensitizer mixed solution soaked with the screen-printed electrode in step 2) to pretreat the malachite green and leave it for 5 minutes;

[0039] 4) After standing still, malachite green an...

Embodiment 2

[0041] The present embodiment provides a kind of malachite green rapid detection method, comprises the steps:

[0042] 1) Add 1 mL of sodium acetate buffer solution with a concentration of 0.1 mol / L and pH=4.5 to 2 μL of 0.1 mol / L sodium sulfite solution, and shake evenly to obtain a sensitizer mixture;

[0043] 2) After connecting the screen-printed electrode to the portable electrochemical workstation, immerse it in the sensitizer mixture prepared in step 1), select the square wave anodic stripping voltammetry, the activation potential window is 0.3V-0.7V, and the voltage increases Volume 4mV, amplitude 0.025V, frequency 15Hz, activate the screen printing electrode;

[0044] 3) After the activation is completed, add a malachite green solution with a concentration of 36ppb to the sensitizer mixed solution soaked with the screen-printed electrode in step 2) to pretreat the malachite green and leave it for 5 minutes;

[0045] 4) After standing still, malachite green and sodium...

Embodiment 3

[0048] The present embodiment provides a kind of malachite green rapid detection method, comprises the steps:

[0049] 1) Add 1 mL of sodium acetate buffer solution with a concentration of 0.1 mol / L and pH=4.5 to 2 μL of 0.1 mol / L sodium sulfite solution, and shake evenly to obtain a sensitizer mixture;

[0050]2) After connecting the screen-printed electrode to the portable electrochemical workstation, immerse it in the sensitizer mixture prepared in step 1), select the square wave anodic stripping voltammetry, the activation potential window is 0.3V-0.7V, and the voltage increases Volume 4mV, amplitude 0.025V, frequency 15Hz, activate the screen printing electrode;

[0051] 3) After the activation is completed, add a malachite green solution with a concentration of 72ppb to the sensitizer mixed solution soaked with the screen-printed electrode in step 2) to pretreat the malachite green and leave it for 5 minutes;

[0052] 4) After standing still, malachite green and sodium ...

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Abstract

The invention discloses a malachite green rapid detection method. According to the malachite green rapid detection method, sodium sulfite or sodium hyposulfite is taken as a sensitizer, a malachite green solution is added into a buffer solution containing the sensitizer, an electrode is immersed into the sensitizer buffer solution containing malachite green, and is allowed to stand for open circuit enrichment, an intermediate is obtained via addition reaction of malachite green with sodium sulfite, enrichment of the intermediate onto the electrode is realized, square-wave anodic stripping voltammetry method is adopted for detection so as to obtain a standard curve used for representing the change of dissolving out current with malachite green concentration, and then square-wave anodic stripping voltammetry method and the standard curve are adopted to determine the concentration of the malachite green solution to be measured, and the malachite green content is obtained. No expensive instrument or reagent is needed in preparation and detection process of the malachite green rapid detection method; cost is low; operation is simple; detection sensitivity is high; detection results arestable; and market application prospect is promising.

Description

technical field [0001] The invention relates to the field of detection and analysis of organic compounds, in particular to a rapid detection method for malachite green. Background technique [0002] Malachite green is a triphenylmethane-type green dye, commonly known as basic green, aniline green, etc., widely used in leather industry, paper industry and textile printing and dyeing industry. At the same time, malachite green is also a bactericidal and insecticide with excellent performance. It has a good killing effect on common diseases of fish, shrimp and other aquatic products, such as saprolegniasis, gill mold and melon worm disease; but peacock Stone green is rapidly metabolized into colorless malachite green in fish. Colorless malachite green has higher toxicity and is easy to remain. At the same time, it has teratogenic and carcinogenic effects. It is a banned drug for aquaculture that is banned by most countries. However, due to the low price and excellent effect of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/38G01N27/26G01N27/30
CPCG01N1/38G01N27/26G01N27/30
Inventor 战艺芳吴学征曾令文韩艳云朱运峰王利华彭磊吴文辉周琦
Owner WUHAN ACADEMY OF AGRI SCI