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Method for preparing Au@Ag-PPY composite nanometer material and L-cysteine double-layer film immobilized glucose oxidase modified electrode

A composite nanomaterial and glucose oxidase technology, which is applied in the field of bioelectrochemical sensors, can solve the problems of positive measurement potential, complicated electrode fabrication, mediator pollution, etc., and achieves short response time, high sensitivity, and good biocompatibility. Effect

Inactive Publication Date: 2015-01-14
甘肃省科学院传感技术研究所
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Problems solved by technology

In this type of biosensor, electron transport has nothing to do with oxygen and other electron acceptors, and does not require the addition of any mediator, which overcomes the measurement potential bias in the presence of mediators, the interference of electroactive substances, the complexity of electrode fabrication, and the mediation of mediators. Disadvantages such as pollution

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  • Method for preparing Au@Ag-PPY composite nanometer material and L-cysteine double-layer film immobilized glucose oxidase modified electrode
  • Method for preparing Au@Ag-PPY composite nanometer material and L-cysteine double-layer film immobilized glucose oxidase modified electrode
  • Method for preparing Au@Ag-PPY composite nanometer material and L-cysteine double-layer film immobilized glucose oxidase modified electrode

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Embodiment 1

[0036] (1) Instruments and reagents used in this example

[0037] Instrument: AUTOLAB PGSTAT 128N modular electrochemical comprehensive test system (Metrohm, Switzerland), electrochemical measurement uses a three-electrode system: enzyme electrode as working electrode, platinum wire electrode as auxiliary electrode, saturated calomel (SCE) electrode as reference electrode. All electrochemical experiments were performed at room temperature.

[0038] Reagent: chloroauric acid (HClO 4 , Shanghai Reagent No. 1 Factory), Sodium Dodecyl Sulfonate (SDS, Sigma), Silver Nitrate (AgNO 3 , Xi’an Chemical Reagent Factory), pyrrole (Py, Shanghai Reagent No. 1 Factory), L-cysteine ​​(Sigma), disodium hydrogen phosphate (Na 2 HPO 4 , Aldrich), sodium dihydrogen phosphate (NaH 2 PO 4 , Aldrich), potassium ferricyanide (K 3 Fe(CN) 6 , Aldrich), sodium chloride (NaCl, Aldrich), high-purity nitrogen (N 2 , Special Gas Center of Lanzhou Institute of Chemical Physics, Chinese Academy of S...

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Abstract

The invention discloses a method for preparing an Au@Ag-PPY composite nanometer material and an L-cysteine double-layer film immobilized glucose oxidase modified electrode. The method comprises the following steps: preparing gold nanoparticles, preparing an Au@Ag-PPY composite nanometer material, treating an electrode, forming an L-cysteine double-layer film, immobilizing the Au@Ag-PPY composite nanometer material, adsorbing glucose oxidase, and performing after-treatment on the modified electrode, thereby obtaining the glucose oxidase modified electrode. According to the Au@Ag-PPY composite nanometer material / L-cysteine double-layer film, direct electron transfer between the glucose oxidase and the electrode is realized, and a high-sensitivity third generation biosensor is successfully constructed.

Description

technical field [0001] The invention relates to the field of bioelectrochemical sensors, in particular to a method for preparing an electrode modified by immobilizing glucose oxidase. Background technique [0002] Electrochemical biosensors refer to biological components (enzymes, antigens, antibodies, hormones, etc.) or the organism itself (cells, organelles, tissues, etc.) ) As a conversion element, a sensor that uses potential or current as a characteristic detection signal for sensing and detection. Because of its fast response, high sensitivity and simple fabrication, it has been widely studied and applied. The biosensor constructed by direct oxidation or reduction reaction of enzyme on the electrode is called the third generation biosensor. In this type of biosensor, electron transport has nothing to do with oxygen and other electron acceptors, and does not require the addition of any mediator, which overcomes the measurement potential bias in the presence of med...

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Application Information

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IPC IPC(8): G01N27/327
Inventor 王艳凤韩根亮李云霞马丽萍宋玉哲左显维
Owner 甘肃省科学院传感技术研究所