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Preparation method of 3D paper-based electrochemical glucose sensor based on PtNPs

A glucose sensor and electrochemical technology, applied in the field of glucose detection, can solve the problems of high cost, large batch-to-batch variation, and inability to monitor continuously, and achieve the effects of good conductivity, lower detection potential, and improved detection sensitivity

Pending Publication Date: 2020-10-16
GUILIN UNIV OF ELECTRONIC TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these products have limitations such as high cost, large batch-to-batch variation, invasiveness, frequent use of disinfection measures to avoid cross-infection, and inability to continuously monitor.

Method used

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  • Preparation method of 3D paper-based electrochemical glucose sensor based on PtNPs
  • Preparation method of 3D paper-based electrochemical glucose sensor based on PtNPs
  • Preparation method of 3D paper-based electrochemical glucose sensor based on PtNPs

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Embodiment 1

[0044] A 3D paper-based electrochemical glucose sensor based on PtNPs, such as figure 1 As shown, it includes two layers of paper-based microfluidic channels, the working electrode layer ( figure 1 a) and the counter / reference electrode layer ( figure 1 b), the working part of the paper-based screen-printed electrode is printed on the hydrophilic region 5, and the contact part is printed on the hydrophobic region 4, with carbon working electrode 1 printed on the working electrode layer and carbon printed on the counter / reference electrode layer Counter electrode 2 and silver / silver chloride reference electrode 3; PtNPs are electrodeposited on the working electrode, and glucose oxidase is immobilized on the hydrophilic region of the counter / reference electrode.

Embodiment 2

[0046] A preparation of a 3D paper-based electrochemical glucose sensor based on PtNPs, comprising the steps of:

[0047] (1) First soak several pieces of Whatman No. 1 filter paper in 0.03 M KIO 4 solution, incubate at 65 °C for 2 h in an incubator to achieve aldylation of the hydroxyl groups on the surface of the filter paper. After the formylation was completed, the filter paper was rinsed twice with fresh deionized water, 1 min each time. Finally, use dry absorbent paper to absorb excess water on the surface of the washed aldehyde-based filter paper, and fully dry it in a desiccator.

[0048] (2) figure 1 a and figure 1 b shows the hydrophilic and hydrophobic regions of the 3D paper-based microfluidic channel respectively. The preparation process of the paper-based microfluidic channel is as follows: take 2.5 mL of SU-8 2007 negative photoresist and pour it on the aldehyde-modified The center position of whatman No. 1 filter paper, under the action of a homogenizer (50...

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Abstract

The invention discloses a preparation method of a PtNPs-based 3D paper-based electrochemical glucose sensor. The method comprises the following steps: preparing a 3D paper-based microfluidic screen-printed electrode comprising a working electrode layer and a counter / reference electrode layer by a photoetching technique and a screen printing technique; and then modifying a working electrode of the3D paper-based microfluidic screen-printed electrode by adopting a platinum nanoparticle electro-deposition method, and then covalently immobilizing glucose oxidase in an aldehyde group hydrophilic region of a counter / reference electrode to prepare the portable 3D paper-based microfluidic glucose sensor. Compared with the prior art, the preparation method of the 3D paper-based microfluidic glucosesensor is simple, convenient and rapid, glucose oxidase is covalently immobilized through acetonylation, and the 3D paper-based microfluidic glucose sensor has good repeatability, selectivity and stability on glucose and can be used for detecting glucose in body surface sweat.

Description

technical field [0001] The invention belongs to the technical field of biomedical sensing, and in particular relates to a preparation method of a PtNPs-based 3D paper-based microfluidic electrochemical glucose sensor and a method for glucose detection. Background technique [0002] Diabetes is one of the three major diseases that threaten our human health. It is particularly important to control diabetes and its complications. The clinical diagnosis of diabetes is mainly judged by measuring the concentration of glucose in the blood. Therefore, the accurate and rapid detection of glucose concentration in the blood is of great significance to the clinical control of diabetes and its complications. At present, the detection of glucose based on electrochemical methods has achieved remarkable results, and thus a commercial electrochemical glucose detector has been developed. However, these products have limitations such as high cost, large batch-to-batch variation, invasiveness,...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 操良丽陈真诚梁永波肖皓霖赵飞骏魏珊珊
Owner GUILIN UNIV OF ELECTRONIC TECH