Method for producing rejuvenation Cordyceps strains

A production method and a technology for Cordyceps militaris are applied in the directions of botanical equipment and methods, horticulture, plant cultivation, etc., to achieve the effects of simple method, fast growth rate and improved biotransformation rate

Inactive Publication Date: 2018-08-07
镇江市天益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the problems referred to above, the object of the present invention is to provide a production method for rejuvenating the Cordyceps militaris bacterial classification, so as to improve the yield of the fruiting body, enhance the active ingredient content of the fruiting body of Cordyceps, and reduce the infectivity when reconnecting the worm body due to long-term artificial cultivation. Reduced resistance to adverse external conditions including low temperature, high temperature or bacterial infection

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0023] The production method of a kind of rejuvenating Cordyceps militaris bacterial classification of the present embodiment is as follows implementation steps:

[0024] (1) Select 100 fresh, live, complete and healthy tussah chrysalis in diapause stage;

[0025] (2) Put the silkworm chrysalis in an ultrasonic cleaning machine for 45 seconds, and remove them after completion;

[0026] (3) Put the removed silkworm chrysalis in 75% alcohol for 5 minutes to sterilize, and remove them after completion;

[0027] (4) Repeatedly wash the silkworm chrysalis for 4 times with a large amount of sterile water, and then dry the surface moisture with sterile filter paper;

[0028] (5) Under aseptic conditions, use a sterile syringe to inject 0.3ml of liquid bacteria in the thorax of the pupal body in sequence;

[0029] (6) Put the inoculated silkworm chrysalis into the incubator to avoid light and cultivate them, and control the temperature at 10°C for 1-3 days, and adjust the temperatur...

Embodiment 2

[0034] The production method of a kind of rejuvenating Cordyceps militaris bacterial classification of the present embodiment is as follows implementation steps:

[0035] (1) Select 200 fresh, live, complete and healthy tussah chrysalis in the diapause period;

[0036] (2) Put the silkworm chrysalis in an ultrasonic cleaning machine for 45 seconds, and remove them after completion;

[0037] (3) Put the removed silkworm chrysalis in 75% alcohol to sterilize for 4 minutes, and remove them after completion;

[0038] (4) Repeatedly wash the silkworm chrysalis for 4 times with a large amount of sterile water, and then dry the surface moisture with sterile filter paper;

[0039] (5) Under aseptic conditions, use a sterile syringe to inject 0.4ml of liquid bacteria in the thorax of the pupal body in sequence;

[0040] (6) Put the inoculated silkworm chrysalis into the incubator to avoid light and cultivate them, and control the temperature at 10°C for 1-3 days, and adjust the tempe...

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Abstract

The invention discloses a method for producing rejuvenation Cordyceps strains. Surface-sterilized live oak silkworm pupa is inoculated with the Cordyceps strains through a sterile syringe, culture isconducted through low-temperature segmental temperature control, and then culture is conducted through light coloring. Since cordyceps militaris strains are not autoclaved, bioactive components in thecordyceps militaris strains are not destroyed, and the strains are effectively rejuvenated. The method has the advantages of being simple, high in success rate, high in biotransformation rate of thestrains after rejuvenation and the like.

Description

technical field [0001] The invention belongs to the field of fungal seed production, and in particular relates to a new technology for cultivating Cordyceps militaris strains. Background technique [0002] Cordyceps militaris fruiting body [Cordyceps militaris] also known as Cordyceps militaris fruiting body or sub-seat, referred to as Cordyceps militaris, Ascomycota subphylum, Ergotaceae, Cordyceps genus. In recent years, artificial cultivation of fruiting bodies has spread all over urban and rural areas, and has become a new industry in my country. [0003] Artificial cultivation of Cordyceps militaris has achieved large-scale production, but in actual production, due to the significant genetic variation of Cordyceps militaris, the strain will degenerate after long-term asexual reproduction and test tube transfer, which is manifested as mycelium not growing when the fruiting bodies are cultured in solid. Turning color, or not germinating to form fruiting bodies after turn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00
Inventor 陈顺志王林高剑萍
Owner 镇江市天益生物科技有限公司
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