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Method for extracting polysaccharide compound from sargassum pallidum

A compound, Sargassum algae technology is applied in the field of polysaccharide extraction to achieve the effects of low production cost, excellent anti-oxidation, and simple extraction process

Inactive Publication Date: 2017-12-15
浦江县美泽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its advantages are: the extraction rate of Sargassum polysaccharide is high, the loss of biological activity is small, and the purity of Sargassum polysaccharide and Sargasso polyphenol is very high. At the same time, the method of the invention has the advantages of short time, large processing capacity, continuous operation and low cost. , the advantages of clean environment, so it is very suitable for industrial applications, but the yield of Sargassum polysaccharides in this method still has room for improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for extracting polysaccharide complexes from Sargassum, comprising breaking cell walls, concentrating and alcoholizing, redissolving to remove proteins, and freeze-drying. The specific operation steps are as follows:

[0019] 1) Take sargassum powder to make algae liquid, the solid-to-liquid ratio of sargassum powder to water is 1:68, ultrasonic crushing, the power is 700w, the time is 14min, and 0.02% of the weight of the algae liquid is added to the active short peptide, the active short peptide The amino acid sequence is QSGFCTSYYSKFCTLYGNLGICLHCSS, in a water bath at 99°C for 2h, centrifuged at 7500r / min for 8min, to remove algae;

[0020] 2) Put the algae liquid into a vacuum concentration tank to vacuum concentrate to 1 / 4 of the original volume, add 92% ethanol solution, the volume ratio of ethanol to concentrated solution is 3:1, and let the ethanol solution stand in a 0.1T magnetic field for 4 hours ;

[0021] 3) Redissolve, add 20% trichloroacetic aci...

Embodiment 2

[0024] A method for extracting polysaccharide complexes from Sargassum, comprising breaking cell walls, concentrating and ethanolizing, redissolving and removing proteins, and freeze-drying. The preferred specific operation steps are as follows:

[0025] 1) Take sargassum powder to make algae liquid, the solid-to-liquid ratio of sargassum powder to water is 1:70, ultrasonically crush, the power is 750w, the time is 14min, add 0.02% active short peptide by weight of algae liquid, active short peptide The amino acid sequence is QSGFCTSYYSKFCTLYGNLGICLHCSS, in a water bath at 100°C for 4h, centrifuged at 8000r / min for 7min, to remove algae;

[0026] 2) Put the algae solution into a vacuum concentration tank to vacuum concentrate to 1 / 4 of the original volume, add 92% ethanol solution, the volume ratio of ethanol to concentrated solution is 4:1, and the ethanol solution stands in a 0.2T magnetic field for 4 hours ;

[0027] 3) Redissolve, add 20% trichloroacetic acid to the solut...

Embodiment 3

[0030] A method for extracting polysaccharide complexes from Sargassum, comprising breaking cell walls, concentrating and alcoholizing, redissolving to remove proteins, and freeze-drying. The specific operation steps are as follows:

[0031] 1) Take Sargassum powder to make algae liquid, the solid-to-liquid ratio of Sargassum powder to water is 1:72, ultrasonic crushing, the power is 580w, the time is 12min, and 0.018% of the weight of the algae liquid is added. Active short peptide, active short peptide The amino acid sequence is QSGFCTSYYSKFCTLYGNLGICLHCSS, in a water bath at 100°C for 3h, centrifuged at 7500r / min for 8min, to remove algae;

[0032] 2) Put the algae liquid into a vacuum concentration tank to vacuum concentrate to 1 / 4 of the original volume, add 90% ethanol solution, the volume ratio of ethanol to concentrated solution is 3:1, and let the ethanol solution stand in a 0.1T magnetic field for 2 hours ;

[0033] 3) Redissolve, add 20% trichloroacetic acid to the...

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Abstract

The invention discloses a method for extracting a polysaccharide compound from sargassum pallidum and belongs to the technical field of extraction of polysaccharides. The polysaccharide extraction method comprises steps as follows: cell wall breaking, concentration, alcohol precipitation, redissolution for protein removal and freeze drying; the cell wall breaking step is implemented as follows: an algae solution is prepared from sargassum pallidum powder, ultrasonic crushing is performed, active short peptides are added for a water bath, centrifugation is performed, and frond is removed. The cell wall breaking rate of the sargassum pallidum is notably increased through ultrasonic waves and the active short peptides, no bad influence is caused to nutrition loss of the sargassum pallidum, and the extracted sargassum pallidum polysaccharides can be applied to healthcare products for improving the immunity and resisting tumor.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for extracting polysaccharide complexes from Sargassum algae. Background technique [0002] Sargassum pallidum is a class of seaweed in brown algae, belonging to Phaeophyta, Fucales, Sargassaceae, Sargassum. Sargassum is warm temperate Sexual seaweed, a perennial plant, so it can be seen all year round. It is abundant in the Yellow Sea and the Bohai Sea coast of my country. It is a common algae with a large output. It is distributed in the Sea of ​​Okhotsk, the Kuril Islands, Japan and North Korea abroad. The habitat of Sargassum requires smooth waves, strong winds and waves, clear water color, high transparency, and sufficient sunlight. It usually grows in stone marshes in the intertidal zone and on rocks at a depth of 1-4 meters in the subtidal zone. The dominant species in this water area is very rich in resources. [0003] In the past 20 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 谢雨欣
Owner 浦江县美泽生物科技有限公司
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