Technique for extracting and purifying oleanolic acid and ursolic acid from sweet tea

A technology of oleanolic acid and process method, applied in organic chemistry, steroidal compounds, etc., can solve the problems of unreported extraction and purification process, and achieve the effects of long extraction time, low efficiency and convenient operation

Active Publication Date: 2017-12-26
桂林市产品质量检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature review, there is no report on the extraction and purification process of oleanolic acid and ursolic acid in sweet tea

Method used

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  • Technique for extracting and purifying oleanolic acid and ursolic acid from sweet tea
  • Technique for extracting and purifying oleanolic acid and ursolic acid from sweet tea
  • Technique for extracting and purifying oleanolic acid and ursolic acid from sweet tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A process for extracting and purifying oleanolic acid and ursolic acid from sweet tea, comprising the steps of:

[0022] (1) Take a sweet tea sample and crush it with a masher, weigh 150g of the sample, add 2 times its weight in absolute ethanol, ultrasonically extract at 25°C for 10min, and filter the extract with suction; wash with 300mL ethanol after suction filtration The residue 1 time, then suction filtration, the suction filtrate is combined with the extract for the first time, the combined extract is concentrated to obtain an extract, the obtained extract is dissolved in 30mL of chloroform, and the brown extract is obtained after concentration and recovery of chloroform;

[0023] (2) Add 30mL of 5% sodium hydroxide solution to the obtained extract, boil for 10min, suction filter while it is hot, put the filtrate in the refrigerator at 5-10℃ for 1h, suction filter, wash with water until neutral; the residue after suction filtration Add 30 mL of water and boil for...

Embodiment 2

[0030] A process for extracting and purifying oleanolic acid and ursolic acid from sweet tea, comprising the steps of:

[0031] (1) Take a sweet tea sample and crush it with a masher, weigh 150g of the sample, add 3 times the weight of absolute ethanol, and extract it by ultrasonic wave at 35°C for 15min, then filter the extract with suction, and then wash the filtered tea with 300mL ethanol The residue was filtered once again with suction, the suction filtrate was combined with the extract for the first time, the combined extract was concentrated to obtain an extract, the obtained extract was dissolved in 30 mL of chloroform, and the brown extract was obtained after concentration and recovery of chloroform;

[0032] (2) Add 30mL of 5% sodium hydroxide solution to the obtained extract, boil for 10min, suction filter while it is hot, put the filtrate in the refrigerator at 5-10℃ for 1h, suction filter, wash with water until neutral; the residue after suction filtration Add 30 m...

Embodiment 3

[0036] A process for extracting and purifying oleanolic acid and ursolic acid from sweet tea, comprising the steps of:

[0037](1) Take a sweet tea sample and mash it with a masher, weigh 150g of the sample, add 4 times the weight of absolute ethanol, extract it ultrasonically at 40°C for 20min, filter the extract, and wash the filtered tea with 300mL ethanol Residue 1 time, then suction filtration, the suction filtrate is combined with the extract for the first time, and the combined extract is concentrated to obtain an extract, the obtained extract is dissolved in 30mL of chloroform, and the brown extract is obtained after concentration and recovery of chloroform;

[0038] (2) Add 30mL of 5% sodium hydroxide solution to the obtained extract, boil for 10min, suction filter while it is hot, put the filtrate in the refrigerator at 5-10℃ for 1h, suction filter, wash with water until neutral; the residue after suction filtration Add 30 mL of water, heat and boil for 10 minutes, a...

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Abstract

The invention discloses a technique for extracting and purifying oleanolic acid and ursolic acid from sweet tea, which includes the following steps: absolute ethyl alcohol is adopted for ultrasonic extraction and suction filtration; combined extract is concentrated, so that an extract is obtained, chloroform is used for dissolving the obtained extract, and after recovering chloroform by concentration, a brown extract is obtained; 5 percent of sodium hydroxide is used for carrying out alkali dissolution and acid precipitation on the extract, and a method, such as silica gel column chromatography separation, is carried out on precipitate, so that high-purity oleanolic acid and ursolic acid are obtained; by adopting a high-performance liquid chromatography method to carry out content determination, the purities of both oleanolic acid and ursolic acid are 90 percent or more, the yield of oleanolic acid is 20mg / 100g or more, and the yield of ursolic acid is 90mg / 100g or more. The technique disclosed by the invention has the characteristics of simple needed equipment, convenience in operation, short extraction time, high extraction efficiency, energy saving, material saving, mild conditions, no active component destruction and the like, provides a feasible source for the extraction of oleanolic acid and ursolic acid from the natural plant, increases the added value of sweet tea, and has important application value and economic and social benefits.

Description

technical field [0001] The invention relates to a method for extracting and purifying plant effective components, in particular to a process for extracting and purifying oleanolic acid and ursolic acid from sweet tea. Background technique [0002] Guangxi sweet tea (Rubus Suavissimus S.Lee) belongs to the genus Rubus of Rosaceae. It is a unique sweet plant in Guangxi. It is often used as tea in the folks. It contains sweet tea, flavonoids, tea polyphenols and other main active ingredients. It has heat-clearing, thirst-quenching, It has the effects of nourishing the kidney, lowering blood pressure and treating diabetes. It is reported in the literature that oleanolic acid and ursolic acid are identified in sweet tea by means of extraction, separation and spectroscopy. Modern studies have shown that oleanolic acid has a wide range of pharmacological effects and biological activities such as anti-HIV, antibacterial, anti-cancer, anti-ulcer, and treatment of osteoporosis. It ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 谭冬明石相莉农彦贤罗星晔梁炜黄健张鸿斌刘保奇吕新印阳文琼彭慎江有辉
Owner 桂林市产品质量检验所
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