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A kind of aerobic denitrifying bacteria and its application

A technology of aerobic denitrifying bacteria and strains, applied in the field of microorganisms, to achieve wide application prospects, strong denitrification ability, fast and effective denitrification speed

Active Publication Date: 2020-07-17
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few domestic reports on the isolation of aerobic denitrifying bacteria with low carbon-to-nitrogen ratio and high salinity.

Method used

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  • A kind of aerobic denitrifying bacteria and its application
  • A kind of aerobic denitrifying bacteria and its application
  • A kind of aerobic denitrifying bacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 Isolation and identification of the aerobic denitrification bacterial strain of the present invention

[0016] 1.1 Medium and method

[0017] 1.1.1 Medium

[0018] Denitrification liquid medium: KNO 3 2g, KH 2 PO 4 0.5g, MgSO 4 0.25g, 1000mL of distilled water, adjust the pH value to 7.0-7.2, then add sodium acetate (control different C / N), and sterilize at 121°C for 20min.

[0019] Denitrification solid medium: Add 15g of agar powder per liter of denitrification liquid medium.

[0020] 1.1.2 Experimental method

[0021] 10 g of surface soil samples were collected from paddy field soil, briefly crushed by a tissue breaker, then added to a 500 mL Erlenmeyer flask containing 100 mL of denitrification liquid medium, and cultured with shaking at 30 °C and 120 rpm for 24 h. After continuous gradient dilution and gradually reducing the carbon-nitrogen ratio of the medium, a strain of bacteria was isolated and named XPS-1.

[0022] Propagate the isolate...

Embodiment 2

[0033] Example 2 Effect of salinity and carbon-nitrogen mass ratio on the aerobic denitrification of bacterial strain XPS-1

[0034] This embodiment explores the influence of carbon-nitrogen ratio and salinity on the denitrification effect of aerobic denitrification strain XPS-1. During the experiment, sodium acetate (CH 3 COONa) as the sole carbon source, potassium nitrate (KNO 3 ) is the only nitrogen source, by adjusting the quality of sodium acetate to change the C / N ratio (mass ratio) in the denitrification liquid medium, control C / N=2 and C / N=3, and regulate the denitrification liquid medium with NaCl Salinity, so that the mass fraction of NaCl is 1.2% and 1.5%, respectively, inoculate the activated bacteria into the denitrification liquid medium according to the inoculum size of 5%, at 25 °C and 120rpm (DO>7mg / L) Under culture, the total nitrogen concentration and total nitrogen removal efficiency in the culture medium were measured every 12 hours. The measurement resu...

Embodiment 3

[0039] The influence of embodiment 3pH on bacterial strain XPS-1 aerobic denitrification

[0040] On the basis of Example 2, the optimum carbon-to-nitrogen ratio (mass ratio) of the aerobic denitrification strain XPS-1 of the present invention is 3, and the optimum salinity is 1.2% NaCl. Therefore, the present embodiment selects C / N =3(KNO 3 as the sole nitrogen source, CH 3 COONa is the only carbon source) and the denitrification medium whose salinity is 1.2%NaCl, adjust the pH value of the denitrification medium to be pH 5, pH 6, pH7, pH 8 and pH 9 respectively, and the bacterial classification after activation is according to 5% of the inoculum was inoculated into aerobic denitrification enrichment medium with different pH, cultivated under the culture conditions of 25°C and 120rpm (DO>7mg / L), and measured the total nitrogen concentration and removal of The total nitrogen efficiency, the measurement results are shown in Table 2.

[0041] Table 2

[0042]

[0043] As ...

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Abstract

The invention discloses an aerobic denitrifying bacteria and application thereof. The aerobic denitrifying bacteria of the present invention, the strain named XPS-1, was preserved in the General Microbiology Center of the China Microbial Strain Preservation Management Committee, and the preservation address was No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date was 2017 September 29, 2009, the deposit number is CGMCC NO.14772. The invention also provides the application of the aerobic denitrifying bacteria in the denitrification of domestic sewage. The aerobic denitrification bacterial strain of the present invention can carry out high-efficiency denitrification at a low carbon-to-nitrogen ratio and a temperature of 25-30°C, and is especially suitable for denitrification and denitrification in the treatment process of urban domestic sewage with a low carbon-nitrogen ratio and a certain salinity. The nitrogen rate can reach up to 100%, the denitrification speed is fast and effective, and it has a wide application prospect in the field of sewage treatment.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an aerobic denitrifying bacteria and its application. Background technique [0002] Denitrification, also known as denitrification process, is a biological process in which nitrate or nitrite is reduced to gaseous nitrogen. Bacteria involved in this process are collectively referred to as denitrifiers. For a long time, denitrification has been regarded as only under anaerobic or anoxic conditions, and denitrifying bacteria can obtain energy while completing respiration by using nitrate or nitrite as electron acceptor. However, in recent years, the phenomenon of aerobic denitrification has been continuously reported. Aerobic denitrification refers to the reduction of NO in the presence of oxygen 3 - -N converts to N 2 the process of. [0003] Aerobic denitrification has the following advantages: (1) denitrification under aerobic conditions makes simultaneous nitrificat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/40C02F101/16
CPCC02F3/34C02F2101/163C02F2101/166C12N1/205C12R2001/40
Inventor 于昌平王寅寒杨晓永胡安谊林清秀黄自文陈国良马聪
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI