Method for the detection of oligonucleotides of 4 to 10 nucleotide monomers in length

A technology of oligonucleotides and nucleotide monomers, applied in the field of biological detection, can solve the problem that small fragments of nucleotides cannot be detected qualitatively and quantitatively

Active Publication Date: 2020-12-22
TARIM UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Existing findings suggest that these small fragments of nucleotides may have important biological roles in vivo, but qualitative and quantitative detection of small fragments of 10 or less nucleotides has not been possible

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  • Method for the detection of oligonucleotides of 4 to 10 nucleotide monomers in length
  • Method for the detection of oligonucleotides of 4 to 10 nucleotide monomers in length
  • Method for the detection of oligonucleotides of 4 to 10 nucleotide monomers in length

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Embodiment Construction

[0048]In order to illustrate the present invention more clearly, the present invention will be further described below in conjunction with preferred embodiments and accompanying drawings. Similar parts in the figures are denoted by the same reference numerals. Those skilled in the art should understand that the content specifically described below is illustrative rather than restrictive, and should not limit the protection scope of the present invention.

[0049] 1. Detection method and principle

[0050] The probe design and detection procedures for detection based on electrophoresis technology are as follows:

[0051] (1) Designing a single-stranded locked nucleic acid probe as the main probe, the single-stranded locked nucleic acid probe is single-stranded DNA or RNA, and the fragment of the single-stranded locked nucleic acid probe combined with the oligonucleotide to be detected has at least one locked nucleic acid monomer;

[0052] The length of the small fragment o...

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Abstract

The invention discloses a detection method for oligonucleotide with 4 to 10 nucleotide monomers. The detection method comprises the following steps: (1) designing a single-chain lock nucleic acid probe as a main probe, wherein the single-chain lock nucleic acid probe is single-chain DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) and a combination segment of the single-chain lock nucleic acid probe and oligonucleotide to be detected at least has one lock nucleic acid; (2) designing an RNA secondary probe as an auxiliary probe; (3) mixing the excessive secondary probes with the main probeand hybridizing and detecting the content of small-segment DNA or small-segment RNA in a detection sample. According to the detection method disclosed by the invention, the lock nucleic acid probe isdesigned according to a base stacking hybridization principle and qualitative and quantitative detection of the oligonucleotide of 10 nucleotides or less is realized.

Description

[0001] Method for the detection of oligonucleotides of 4 to 10 nucleotide monomers in length technical field [0002] The invention relates to the field of biological detection. More specifically, it relates to methods for the detection of oligonucleotides of 4 to 10 nucleotide monomers in length. Background technique [0003] Oligonucleotides with a fragment length less than or equal to 10 nt (nucleotides) naturally exist in organisms (such as abortive transcripts produced at the initiation of transcription and small fragments of nucleotides produced after the degradation of long fragments of nucleotides ). Existing findings suggest that these small fragments of nucleotides may have important biological roles in vivo, but qualitative and quantitative detection of small fragments of nucleotides of 10 or less has not been possible. Contents of the invention [0004] One object of the present invention is to provide a method for designing locked nucleic acid probes based o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6816
CPCC12Q1/6816C12Q2537/162
Inventor 秦少伟赵利峰
Owner TARIM UNIV
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