Unlock instant, AI-driven research and patent intelligence for your innovation.

Carbon dioxide anesthesia method for marine grouper

A carbon dioxide, grouper technology, applied in fish farming, climate change adaptation, application, etc.

Inactive Publication Date: 2018-08-21
GUANGDONG OCEAN UNIVERSITY
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, except for anesthetics, there is no report on the carbon dioxide anesthesia method of seawater grouper. Therefore, providing a carbon dioxide anesthesia method with low cost and high survival rate is of great significance to the market development of seawater grouper

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Carbon dioxide anesthesia method for marine grouper
  • Carbon dioxide anesthesia method for marine grouper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A carbon dioxide anesthesia method for seawater grouper, the process is as follows:

[0029] (1) Select 50 pearl gentian groupers (average weight 550g) of uniform size and put them into the laboratory preservation transportation system (the preservation transportation system is equipped with cyclone protein separator, biomechanical filter, refrigerator , PP holding pool with a volume of 1 cubic meter). The fish body is kept alive in the transportation system at 25°C and 330L of naturally purified seawater, and is kept for 2 hours without food.

[0030] (2) Preparation of anesthesia solution: Take a 50L plastic box, add 15L of fresh cultured seawater, continuously oxygenate, put in CO 2 Gas inflatable stone, open CO 2 Gas, keep the concentration of carbon dioxide in the water at 140mg / L, make the pH of the water reach 5.6, and lower the CO 2 The gas flow keeps the pH value of the water stable and keeps the dissolved oxygen in the water at 7.0-7.5mg / L.

[0031] (3) Pu...

Embodiment 2

[0034] A carbon dioxide anesthesia method of seawater grouper, its process is the same as embodiment 1, difference is that the average body weight of pearl gentian grouper is 393g.

[0035] It takes about 9.7 minutes for the pearl gentian grouper to enter the anesthesia state from the time it is put into the anesthesia solution; the resurrection time is 2.9 minutes.

Embodiment 3

[0037] A carbon dioxide anesthesia method of seawater grouper, its process is the same as embodiment 1, difference is that the average body weight of pearl gentian grouper is 737g, the pH value of anesthesia solution is 5.65, and the carbon dioxide concentration is 130mg / L.

[0038] The time required for pearl gentian grouper to enter the anesthesia state was 16.8 minutes from the time it was placed in the anesthesia solution; the resurrection time was 3.5 minutes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a carbon dioxide anesthesia method for marine grouper. The method includes step S1 of causing fresh marine grouper to be subjected to feed deprivation and temporary rearing for2-6 h; step S2 of preparing anesthesia liquid: taking seawater, continuously injecting the seawater with oxygen and carbon dioxide to make the pH of the water body reach 5.6 to 5.8 and make the concentration of carbon dioxide be 70-140 mg / L; step S3: transferring the marine grouper having undergone feed deprivation and temporary rearing into the anesthesia liquid in step S2 for soaking, wherein when the fish body turns on its side and has no stress response to irritation, the marine grouper enters an anaesthetic state. The method can make the marine grouper quickly enter the anaesthetic state,it is ensured that the marine grouper in the anaesthetic state keeps a high survival rate for more than 7 h without water, the marine grouper only needs to be placed in fresh seawater, so that the marine grouper can be restored to be normal within 15 min, the fish body muscles and the pH of blood are not affected, and no side effect occurs on the fish body. The method is suitable for operations such as marine grouper breeding, surgical operations in circulation, inspection sampling and waterless transportation.

Description

technical field [0001] The invention belongs to the field of seawater grouper anesthesia, and more specifically relates to a carbon dioxide anesthesia method for seawater grouper. Background technique [0002] Seawater grouper is widely distributed in tropical and subtropical waters, and is a rare and valuable fish at the bottom of warm water offshore. Its suitable temperature range is 15-34°C, and the optimum water temperature is 22-28°C; its suitable salt range is wide, and it can survive in sea areas with a salinity above 10‰. Seawater grouper is a carnivorous and fierce fish that preys on benthic crustaceans, various small fishes and cephalopods in a surprise manner. Seawater grouper is rich in nutrition, tender and white, similar to chicken, and known as "sea chicken". Because of its high polyunsaturated fatty acid and protein content, it has high use value and health care value, and is promoted by Hong Kong and Macao as the Chinese food. One of the four famous fish. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 范秀萍秦小明章超桦陈建平朱乾峰
Owner GUANGDONG OCEAN UNIVERSITY