Composition comprising botulinum toxin
A kind of botulinum toxin, composition technology, applied in the field of applying the composition of the present invention, spasticity and/or wrinkles, treatment or prevention of dystonia, can solve problems such as blepharoptosis
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example 1
[0125] Two subunits of the composition are prepared.
[0126] Subunit 1: A lyophilizate containing 50 units of botulinum neurotoxin type A, 25 mg human serum albumin (HSS) and 1.18 mg sucrose was reconstituted with 1 ml 0.9% saline.
[0127] Subunit 2: Provide a solution containing 1 ml of water, 14 mg of non-cross-linked hyaluronic acid with an average molecular weight of about 3.8 kD, 34 mg of mannitol, 3.0 mg of glycerol, 0.6 mg of phosphate buffer.
[0128] The subunits were mixed such that 1 ml of the resulting aqueous compositions each contained:
[0129]
[0130] Reference example
[0131] The reference composition was obtained by reconstituting a lyophilizate containing 50 units of botulinum neurotoxin type A, 25 mg of human serum albumin (HSS) and 1.18 mg of sucrose with 1 ml of 0.9% saline.
[0132] Thus, the resulting aqueous compositions each contain:
[0133]
[0134] 2. Comparison of efficacy
[0135] 2.1 Each group of 10 mice was injected into the righ...
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Abstract
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