Cartilage tissue mincing and enzymatic hydrolysis digestion integrated apparatus as well as cartilage tissue processing method

A cartilage tissue and enzymatic hydrolysis technology, applied in cell dissociation methods, bone/connective tissue cells, biochemical equipment and methods, etc., can solve the problem of unsatisfactory number of cartilage cells, affecting the efficiency of cell culture experiments, and insufficient enzymatic digestion and other issues, to achieve the effect of ensuring a sterile environment, avoiding external contamination, and simple steps

Active Publication Date: 2018-09-04
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The entire enzymatic hydrolysis process is in a static state, and the contact between the enzyme and cartilage tissue fragments and chondrocytes is insufficient, resulti...

Method used

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  • Cartilage tissue mincing and enzymatic hydrolysis digestion integrated apparatus as well as cartilage tissue processing method
  • Cartilage tissue mincing and enzymatic hydrolysis digestion integrated apparatus as well as cartilage tissue processing method
  • Cartilage tissue mincing and enzymatic hydrolysis digestion integrated apparatus as well as cartilage tissue processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Reference figure 1, The cartilage tissue mincing device of this program includes a first motor 2, a transmission mechanism, a cam set 6, a knife holder 1, a bevel gear 7 and a vessel 17. The transmission mechanism includes a transmission belt 3 driven by the first motor 2, a gear set A4 and a gear set B8; 302 outer peripheral belt 303; gear set A 4 includes gear A 401 and gear A'402 and the two cooperate with each other; 401 is located on the same connecting shaft A501, and both gear A'402 and gear B'802 are located on the gear set connecting shaft 16. The cam group 6 includes a cam A 601 and a cam B 602 , the cam A 601 is located on the same connecting shaft A 501 as the gear A 401 and the slave transmission wheel 302 , and the cam B 602 and the gear B 801 are located on the same connecting shaft B 502 . The knife rest 1 comprises two vertical bars 101 parallel to each other, a cross bar 102 that is positioned at the top of the two vertical bars 101 and connects the ...

Embodiment 2

[0046] Reference figure 1 and gesture figure 2 , compared with the structure in Example 1, the cartilage tissue chopping, enzymolysis and digestion integrated device of this embodiment is also provided with an enzymolysis and digestion device, including a second motor 13, a coupling 14 connected with the second motor and The second cam 15, the bottom of the hemispherical container 1701 is also provided with a fixed surface 1702, the second cam 15 is positioned at the bottom of one side of the fixed surface 1702 and the top is connected with the fixed surface 1702; the outside of the bottom of the hemispherical container 1701 is provided with a screw hole ( See figure 2 , not marked with reference signs) and does not penetrate the bottom of the container, the top side of the fixed surface 1702 is provided with bolts (see figure 2 , not marked with reference numerals), the bolt cooperates with the screw hole at the bottom of the hemispherical container, and the hemispherica...

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PUM

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Abstract

The invention discloses a cartilage tissue mincing and enzymatic hydrolysis digestion integrated apparatus. The cartilage tissue mincing and enzymatic hydrolysis digestion integrated apparatus comprises a first motor, a transmission mechanism, a cam set, a knife rest, a bevel gear and a vessel, when the first motor is turned on, the transmission mechanism drives the cam set and the bevel gear to rotate, when the vessel is horizontally rotated under driving of the bevel gear, and the knife rest is subjected to reciprocating vertical motion under driving of the cam set; and the cartilage tissuemincing and enzymatic hydrolysis digestion integrated apparatus also comprises a safety cabinet, and an ultraviolet lamp is arranged at the top of the safety cabinet. The method comprises the following steps: apparatus disinfecting, tissue mincing, enzymatic hydrolyzing, and ending. The apparatus and the cartilage processing method can mince the cartilage tissue to cartilage particles with a sizebeing 0.1 cm<3>, the vessel is swung during an enzyme slaking process, the cartilage tissue can be fully digested, the suspension cartilage cells are formed, the manpower and time can be saved, the efficiency is increased, extraneous pollution source can be effectively avoided during a whole operation process, the step is simple, labor saving and time saving are realized, aseptic environment is guaranteed, normalizing operation is realized, and the processing efficiency is obviously increased.

Description

technical field [0001] The invention belongs to the technical field of medical experimental equipment, in particular, the invention relates to an integrated device for tissue chopping and enzymatic digestion. Background technique [0002] Osteoarthritis (OA) is a common aging disease caused by multiple factors causing articular cartilage fibrosis, chapping, ulceration, and loss. Clarifying the pathogenesis of osteoarthritis and exploring effective and economical early intervention methods are the focus of the current research process. Since chondrocytes are the only cell type in cartilage tissue, current in vitro experiments are based on how chondrocytes produce a series of responses to external factors. Therefore, extracting chondrocytes from cartilage tissue of patients undergoing knee replacement surgery is a prerequisite for subsequent experiments. [0003] In order to obtain chondrocytes, the cartilage tissue needs to be minced to a suitable size, and then the cartila...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0655C12N2509/00C12N2509/10
Inventor 雷光华谢名晟曾超李辉李宇晟杨原蘅
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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