An electrochemical sensor for rapid detection of unstable substances and a method for rapid quantitative detection of ATP using the same
An electrochemical and unstable technology, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve problems such as low accuracy and unstable properties, and achieve accelerated molecular diffusion, high-sensitivity content detection, and biocompatibility The effect of improving the conductivity and conductivity
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[0037] The method for rapid quantitative detection of ATP of the present invention is realized by the following steps:
[0038] (1) Mix anti-ATP aptamers with a concentration of 1 μmol / L and their complementary strands into tris(2-carboxyethyl)phosphine (abbreviated as TCEP, 98+%, purchased from Sigma) containing 1 mmol / L and 1mol / L NaClO 4 , in a phosphate buffer solution with a pH of 7.4, heated to 80°C, and slowly cooled to room temperature to obtain dsDNA;
[0039] The anti-ATP aptamer and its complementary chain were synthesized by Shanghai Sangon Bioengineering Co., Ltd.
[0040] The sequence of the anti-ATP aptamer is:
[0041] 5'-ferrocene-C6-ACCTGGGGGAGTATTGCGGAGGAAGGTTT-C6-SH-3';
[0042] The sequence of the complementary strand is:
[0043] 5'-ACCTTCC TCCGC AATAC TCCCC CAGGT-3'.
[0044] (2) Ultrasonically clean the Pt disk ultramicroelectrode with ultrapure water for 5 minutes, use the Pt disk ultramicroelectrode as the working electrode, and put in a solution...
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