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A kind of dna nano drug carrier with high drug loading effect, preparation method and application
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A nano-drug carrier and high drug-carrying technology, applied in the field of biomedicine, can solve the problems of DNA nano-drug carrier, poor controllability of shape and size, and inability to be used as drug carrier, etc., so as to improve sequence reuse and morphology. and uniform size, improving the effect of drug loading
Active Publication Date: 2021-03-23
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY +1
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Problems solved by technology
DNA origami technology is to use the structure of DNA molecule itself and the specific combination between molecules to fold DNA into various 2D and 3D structures like "paper". However, the nano drug carrier constructed by DNA origami has uniform shape and size and high complexity. , the number of short DNA strands required is large, and the synthesis cost is high
DNA module assembly decomposes the target structure into smaller structural units, and uses the pre-designed sticky ends of each structural unit to connect each unit to form a complete DNA nano-drug carrier, but the controllability of shape and size is poor. The uniformity of nano-drug carriers is not good, and even the length of nano-drug carriers exceeds the size of cells, so it cannot be used as a drug carrier
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Embodiment 1
[0026] A DNA nano drug carrier with high drug loading effect, the DNA nano drug carrier is prepared from two DNA strands shown in SEQ ID NO.1 and SEQ ID NO.2, or prepared from SEQ ID NO.3, SEQ ID NO. It is prepared from the two DNA strands shown in ID NO.4, or prepared from the two DNA strands shown in SEQ ID NO.5 and SEQ ID NO.6.
[0027] A method for preparing the above-mentioned DNA nano drug carrier with high drug loading effect, comprising the following steps:
[0028] S1, prepare the following paired DNA strands (the DNA sequences involved in the present invention are all synthesized by Shanghai Sangong Biotechnology Co., Ltd.), for future use:
[0029] Staple 1: 5'-aatcggaaatccgttcttatcaacgcaagcggacgagtgctg-3', as shown in SEQ ID NO.1,
[0030] Staple 2:5'-cgcttgcgttcagcactcgtcatttccgattgataagaacgg-3', as shown in SEQ ID NO.2;
[0031] Prepare 1×TEA buffer, and 1×TEA buffer contains 30mmol / L Mg 2+ , Tris of 20mmol / L, EDTA of 2mmol / L, pH 7.6, solvent is ultrapure wate...
Embodiment 2
[0036] The only difference between embodiment 2 and embodiment 1 is that the two DNA strands are replaced by:
[0037] Staple 3:5'-gtttacttagggatggtacgaactcaacgcac-3', as shown in SEQ ID NO.3,
[0038] Staple 4:5'-agttcgtagtgcgttgaagtaaacccatccct-3', as shown in SEQ ID NO.4;
[0039] The remaining preparation steps and AFM characterization methods are the same as in Example 1. Figure 4 It is an atomic force microscope scanning image of the tubular DNA nano drug carrier synthesized in Example 2 at a scale length of 1.0 μm.
Embodiment 3
[0041] The only difference between embodiment 3 and embodiment 1 is that the two DNA strands are replaced by:
[0042] Staple 5: 5'-aatcggaaatccgttcttatcaacgcaagcggacgagtgctg-3', as shown in SEQ ID NO.5,
[0043] Staple 6:5'-gcttgcgttcagcactcgtcctttccgattgataagaacgga-3', as shown in SEQ ID NO.6;
[0044] The remaining preparation steps and AFM characterization methods are the same as in Example 1. Figure 5 It is an atomic force microscope scanning image of the tubular DNA nano drug carrier synthesized in Example 3.
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Abstract
The invention belongs to the technical field of biological medicines and particularly relates to a DNA nano-drug carrier with a high drug-carrying effect and a preparation method and application of the DNA nano-drug carrier. The DNA nano-drug carrier is prepared from two DNA chains represented by SEQ ID NO.1 and SEQ ID NO.2, or is prepared from two DNA chains represented by SEQ ID NO.3 and SEQ IDNO.4, or is prepared from two DNA chains represented by SEQ ID NO.5 and SEQ ID NO.6 and can be applied to carrying type anti-cancer drugs. The steps for synthesizing the DNA nano-drug carrier are simple, the DNA nano-drug carrier is low in cost and uniform in shape and size, and the drug carrying effect can be effectively improved.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a DNA nano drug carrier with high drug loading effect, a preparation method and an application. Background technique [0002] In recent years, DNA nanotechnology has become a hot field of research at home and abroad, and hundreds of articles on DNA nanotechnology have been published in top international journals such as Nature and Science. DNA is not just the code of life, it is a universal component for making nanoscale building blocks and devices. Through modern biotechnology, according to the simple nucleic acid base pairing rules, DNA molecular chains can be synthesized artificially, which opens up a broad new world for the application of DNA, and is not limited to the field of biological evolution in nature. Using structural DNA nanotechnology, precise nanostructures can be realized through the excellent self-assembly and recognition capabilities of DNA mole...
Claims
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