Novel bacterium detection method

Abstract

The invention discloses a novel bacterium detection method, which comprises the following steps of (1) preparing a test solution; (2) measuring 1ml of the test solution prepared in the step (1); injecting the test solution into a culture vessel; performing uniform shaking; (2) sucking 1ml of culture medium by a disposable sterilized injector; injecting the culture medium into the culture vessel inthe step (2); performing sealed uniform shaking; (4) putting the prepared culture vessel in a culture box with the temperature being 17 to 23 DEG C and the relative humidity being 40 to 47 percent tobe cultured for 168 hours; recording the bacterium number. The used culture vessel and culture medium are sterilized and can be directly used after being opened; the detection sample and the liquid culture medium can be added in normal temperature ordinary environment; the requirements on environment and personnel are very low.

Description

technical field [0001] The invention belongs to the field of bacteria detection, in particular to a novel bacteria detection method. Background technique [0002] According to the YY0572-2015 standard of "Water for Hemodialysis and Related Therapeutics", the plate method and membrane filtration method are usually used to detect the number of microorganisms in the water for hemodialysis and related treatments. The plate method includes the pouring plate method and the coating plate method. The steps of this method are cumbersome and the process is easy to be contaminated and affect the accurate counting. The membrane filtration method is more cumbersome to operate and has higher requirements for the test product. In addition, whether it is the plate method or the membrane filtration method, it is necessary to conduct experiments on a clean bench, make a culture medium or add test samples, which requires relatively high requirements for the test environment, professional leve...

AI Technical Summary

Problems solved by technology

The plate method includes the pouring plate method and the coating plate method. The steps of this method are cumbersome and the process is easy to be contaminated and affect the accurate counting. The membrane filtration method is more cumbersome to operate and has higher requirements for the test product

In addition, whether it is the plate method or the membrane filtration method, it is necessary to carry out the experiment on the ultra-clean bench, make the culture medium or add the test sample, which requires relatively high requirements for the test environment, professional level of personnel and equipment

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