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A kind of preparation method of soluble microneedle loaded with Chinese cobra neurotoxin

A Chinese cobra, neurotoxin technology, applied in the field of microneedle, can solve the problems of low dose, dermal tissue obstruction, and inappropriate high dose drugs

Active Publication Date: 2020-10-30
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But precisely coating the microneedles is difficult, and the amount of drug that can be delivered per dose is quite low, making it unsuitable for high-dose drug delivery
Although hollow microneedles can be used for continuous drug delivery, each needle has only one outlet and may be blocked by dermal tissue
In addition, there is a potential risk that the tips of these three types of microneedles could break within the skin

Method used

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  • A kind of preparation method of soluble microneedle loaded with Chinese cobra neurotoxin
  • A kind of preparation method of soluble microneedle loaded with Chinese cobra neurotoxin
  • A kind of preparation method of soluble microneedle loaded with Chinese cobra neurotoxin

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Embodiment Construction

[0030] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be construed as limiting the present invention.

[0031] 1. Raw materials and instruments

[0032] Chinese cobra neurotoxin (NT, purity > 97%, Yunnan Longfenggu Biopharmaceutical Co., Ltd.); chondroitin sulfate (CS, Zhengzhou Outai Chemical Products Co., Ltd.); polyvinylpyrrolidone (PVP k30, Sigma-Aldrich, USA) ; carboxymethyl cellulose (CMC, Sinopharm Chemical Reagent Co., Ltd.); fluorescein isothiocyanate (FITC, American Sigma-Aldrich Company); >99%, self-made); trypan blue (Shanghai Yuanye Biotechnology Co., Ltd.); experimental water was deionized water, and other reagents were of analytical grade.

[0033] Microneedle mold (Taizhou Weikai Biotechnology Co., Ltd.); HF-50 digital push-pull gauge, HLX-S push-pull gauge test rack (PACK, USA); B008 portable microscope (Shenzhen Chaoyan Technology Co., Ltd.); TL6R Vertical low...

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Abstract

The invention discloses a Chinese cobratoxin loaded soluble microneedle preparation method. The method comprises the steps: (1) weighing CS and PVP according to a certain proportion, evenly mixing theCS and the PVP and then adding the certain amount of Chinese cobratoxin water solution to obtain a medicine containing matrix solution; (2) injecting the medicine containing matrix solution into a microneedle mould, then performing first centrifugation on the microneedle mould at the temperature of 4 DEG C, removing and collecting the medicine containing matrix solution on the surface of the microneedle mould, then drying for 1h and taking out for standby application; (3) microneedle backing layer preparation: weighing the appropriate amount of a backing material, dissolving the backing material into deionized water, evenly mixing, injecting into the microneedle mould with the medicine containing matrix solution, then performing second centrifugation on the microneedle mould at the temperature of 4 DEG C, drying and forming and finally demoulding to obtain a Chinese cobratoxin loaded soluble microneedle (DMN-NT).

Description

technical field [0001] The invention belongs to the technical field of microneedles, and in particular relates to a preparation method of a soluble microneedle loaded with neurotoxin of cobra sinensis. Background technique [0002] Transdermal drug delivery system (TDDS) has been widely used in drug delivery, and has the following advantages: first, TDDS is one of the preferred drug delivery methods for patients due to its convenience; second, TDDS can maintain drug release and Reducing the frequency of dosing, while the sustained release of the drug can also reduce the fluctuation of plasma drug levels and reduce adverse reactions; finally, transdermal delivery, as a painless way of administration, has good patient compliance. Although TDDS has so many advantages, it is only suitable for drugs with low molecular weight, suitable oil-water partition coefficient and low melting point. This is mainly because the stratum corneum (Stratum corneum, SC), the outermost layer of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K9/00A61K47/36A61K47/32A61K47/38A61M37/00
CPCA61K9/0021A61K38/1703A61K47/32A61K47/36A61K47/38A61M37/0015A61M2037/0053
Inventor 李范珠陶成浩郑红月郑杭生姚文栋黄安皓张燕
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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