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A Novel Molecular Marker and Its Application in Preparation of a Kit for Diagnosis and Prognosis of Renal Clear Cell Carcinoma

A technique for renal clear cell carcinoma and molecular markers, applied in the field of biological detection, can solve problems such as variable behavior and uncertain prognosis, and achieve high sensitivity, good specificity, high clinical use and promotion value

Active Publication Date: 2020-07-14
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the histological behavior of clear cell renal cell carcinoma is variable, and the prognosis is uncertain. Nearly 30% of patients have postoperative tumor recurrence

Method used

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  • A Novel Molecular Marker and Its Application in Preparation of a Kit for Diagnosis and Prognosis of Renal Clear Cell Carcinoma
  • A Novel Molecular Marker and Its Application in Preparation of a Kit for Diagnosis and Prognosis of Renal Clear Cell Carcinoma
  • A Novel Molecular Marker and Its Application in Preparation of a Kit for Diagnosis and Prognosis of Renal Clear Cell Carcinoma

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Embodiment Construction

[0015] The present invention will be further elaborated below through specific examples. It should be noted that the following descriptions are only for explaining the present invention, and do not limit its content.

[0016] The method for obtaining the molecular marker of the present invention is as follows:

[0017] 1. Download 620 tRNA gene annotation information and their sequences from the GtRNAdb (http: / / gtrnadb.ucsc.edu / genomes / eukaryota / Hsapi19) database. Annotations and sequence information for newly constituted CCA-tRNAs were obtained by adding "CCA" at the 3′ end of the mature tRNA sequence. Then, starting from the 5' or 3' end of the CCA-tRNAs sequence, all fragment sequences in the range of 15-30 nt were obtained. After further merging the same sequences, 8681 fragment sequences and 9285 fragment sequences from the 5' end of CCA-tRNAs were finally obtained. A fragment sequence from the 3′ end of CCA-tRNAs. In addition, we used the bedtools tool to obtain the an...

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Abstract

The invention discloses application of a novel molecular marker to the preparation of a kit for diagnosis and prognosis of renal clear cell carcinoma. The RNA sequence of the novel molecular marker isshown in SEQ ID NO: 1 to SEQ ID NO: 57. A mathematical model for diagnosis of renal clear cell carcinoma is constructed by taking the marker as a base; the model is high in sensitivity and good in specificity, AUC can be as high as 0.997, and the diagnosis effect is good. In addition, 57 tRF fragments can serve molecular markers for classifying renal clear cell carcinoma and predicating the survival periods of patients; in test data, a renal clear cell carcinoma tumor sample is clustered into 3 subtypes according to rRFs expression, and survivorship curve analysis shows that the survival periods of the subtypes have obvious difference. The novel tRF molecular marker has excellent diagnosis index characteristic, can be effectively applied to the diagnosis, classification and prognosis of renal clear cell carcinoma, and has high clinical application and promotion values.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a novel molecular marker and its application in preparing a kit for diagnosis and prognosis of renal clear cell carcinoma. Background technique [0002] Renal cell carcinoma (renal cell carcinoma) is one of the most lethal tumors in the urinary system. It is a malignant tumor originating from the urinary tubular epithelial system of the renal parenchyma. It is also known as renal adenocarcinoma and is one of the most common tumors. Its incidence and Mortality accounts for about 2-3% of all tumors. Kidney clear cell renal carcinoma (KIRC) is the most common subtype of renal cancer, accounting for about 70-80%. About 102,000 patients worldwide will die from it. Clear cell renal cell carcinoma is a tumor that develops relatively slowly, and its early symptoms often lack specificity. Many clear cell renal cell carcinomas are found in the middle and late stages when the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11G16B30/10G16B50/30
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178G16B20/00
Inventor 刘鹏渊王平孙喜伟陆燕周莉媛
Owner ZHEJIANG UNIV