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Method for increasing the content of fructosylated mangiferin in plant extract

A plant extract, fructosylation technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as poor solubility, limited formulation development, and many by-products.

Active Publication Date: 2021-07-27
FOOD IND RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although mangiferin has a wide range of pharmacological activities, its solubility is very poor, which seriously limits the development of its preparations. For the convenience of application, it is necessary to improve the solubility and bioavailability of mangiferin
[0005] For natural active ingredients with complex structures, the use of chemical synthesis for structural modification has disadvantages such as low yield, poor reaction specificity, and many by-products. Can make up for the deficiency of chemical synthesis

Method used

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  • Method for increasing the content of fructosylated mangiferin in plant extract
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  • Method for increasing the content of fructosylated mangiferin in plant extract

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Embodiment

[0040] Materials and Methods

[0041] First, materials and methods

[0042] 1. Culture of medium formula and strain

[0043] (1) Lactic acid bacteria medium (MRS medium)

[0044] MRS medium formula: 1G Montho Peptone No. 3,1g Beef Extract, 0.5 g of yeast extract (Yeast Extract), 2 g of DEXTROSE, 0.1Gtween 80 surfactant, 0.2 g of ammonium citrate, 0.5 g of CH 3 COONA, 10mg Mgso 4 · 7h 2 O, 5mg mnso 4 · H 2 O, 0.2g K 2 HPO 4 Subsequently hydrating allows its volume to reach 100 ml, and the pH is adj to 6.5 to perform high pressure sterilization. After the strain is activated in the culture medium, the amount of strains inoculum is 10% of the medium volume. The growth temperature is 37 ° C, pH 6.5, and sedation (0 rpm) culture, OD of the bacterial liquid. 600 At 10 o'clock, centrifugation was removed to give the supernatant.

[0045] (2) Bacterial medium (NUTRIENT medium)

[0046] 0.3 g beef extract, 0.5 g of PEPTONE, and then hydrophilic reached 100ml, and the pH is 7.0 to perform h...

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Abstract

A use of lactic acid bacteria (Lactobacillus plantarum) with a preservation number of BCRC 11697 to perform a biotransformation reaction of mangiferin and a glycosyl donor to produce fructosylated mangiferin. A specific embodiment of the use includes a method for increasing the content of fructosylated mangiferin in the mango peel extract.

Description

[0001] The present invention requires priority to China Taiwan Patent Application No. 106107637, filed on March 8, 2017, is incorporated herein by reference. Technical field [0002] The present invention is a method of increasing the content of fructoside mangosine in the mango extract in the mango extract, and a lactic acid bacteria (Lactobacillus Plantarum) with a deposit number BCRC 11697 (Lactobacillus Plantarum) Application of biological transforming reactive results of mango and glycosyl donors. Background technique [0003] Mango is a tetrahydroxypyrone carbone, a bisbenzopopyrone flavonoid compound, existed in a variety of plants, such as mango trees, tonar trees, Northeast Dragon, and aim. Molecular formula: C 19 Hide 18 O 11 , Molecular weight: 422.3. Mango is a variety of bioactivity and pharmacological effects, such as antioxidants, anti-tumor, immune regulation, anti-diabetic, anti-inflammatory, etc. (Deng Jiagang, Journal of Changchun University of Technology, 2008,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/58C12R1/25
CPCC12P19/58
Inventor 洪怡芳傅威昌蔡孟贞赖进此
Owner FOOD IND RES & DEV INST